Molecular characterization and crystallization of Diocleinae lectins

被引：63

作者：

Calvete, JJ

Thole, HH

Raida, M

Urbanke, C

Romero, A

Grangeiro, TB

Ramos, MV

da Rocha, IMA

Guimaraes, FN

Cavada, BS

机构：

[1] CSIC, Inst Biomed, E-46010 Valencia, Spain

[2] Hannover Med Sch, Zentrum Kinderhielkunde & Humangenet, D-30623 Hannover, Germany

[3] Niedersachs Inst Peptid Forsch Gmbh, D-30625 Hannover, Germany

[4] Hannover Med Sch, D-30623 Hannover, Germany

[5] CSIC, Ctr Invest Biol, E-28006 Madrid, Spain

[6] Univ Fed Ceara, Dept Biol, BR-60451970 Fortaleza, Ceara, Brazil

[7] Univ Fed Ceara, Dept Bioquim & Biol Mol, Biomol Lab, BR-60451970 Fortaleza, Ceara, Brazil

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1999年 / 1430卷 / 02期

关键词：

lectin; diocleinae tribe; amino acid sequence; mass spectrometry; analytical ultracentrifugation; crystallization; (legumes);

D O I：

10.1016/S0167-4838(99)00020-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular characterization of seven Diocleinae lectins was assessed by sequence analysis, determination of molecular masses by mass spectrometry, and analytical ultracentrifugation equilibrium sedimentation. The lectins show distinct pH-dependent dimer-tetramer equilibria, which we hypothesize are due to small primary structure differences at key positions. Lectins from Dioclea guianensis, Dioclea virgata, and Cratylia floribunda seeds have been crystallized and preliminary X-ray diffraction analyses are reported. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：367 / 375

页数：9

共 36 条

[1] PROTEIN-CARBOHYDRATE INTERACTION .7. PHYSICAL AND CHEMICAL STUDIES ON CONCANAVALIN A HEMAGGLUTININ OF JACK BEAN [J].