Microsatellite polymorphism in tsetse flies (Diptera: Glossinidae)

被引:33
作者
Luna, C [1 ]
Bonizzoni, M [1 ]
Cheng, QY [1 ]
Robinson, AS [1 ]
Aksoy, S [1 ]
Zheng, LB [1 ]
机构
[1] Yale Univ, Sch Med Epidemiol & Publ Hlth, New Haven, CT 06520 USA
关键词
Glossina; microsatellite; tsetse fly; sleeping sickness; population genetics;
D O I
10.1603/0022-2585-38.3.376
中图分类号
Q96 [昆虫学];
学科分类号
摘要
In sub-Saharan Africa, tsetse flies are the vectors of trypanosomes. the causative agents of sleeping sickness in humans and nagana in animals. Certain wild populations of the palpalis group exhibit intraspecific variation and are suspect of manifest differences in vectorial capacity. The current study reports the identification of 13 polymorphic microsatellite loci from Glossina palpalis palpalis Robinean-Desvoidy. The majority of these markers amplify corresponding loci from the related species C. p, gambiensis Vanderplank, G. f. fuscipes Newstead, and G. tachinoides Westwood. Only seven of 13 loci were amplified from G. austeni Newstead. Genetic variability was estimated in one field population of G. p. gambiensis. These results confirmed that microsatellite markers may be used to examine the subpopulation structure of tsetse flies.
引用
收藏
页码:376 / 381
页数:6
相关论文
共 33 条
[1]   A simple new method for estimating null allele frequency from heterozygote deficiency [J].
Brookfield, JFY .
MOLECULAR ECOLOGY, 1996, 5 (03) :453-455
[2]   Concordant evolution of a symbiont with its host insect species:: Molecular phylogeny of genus Glossina and its bacteriome-associated endosymbiont, Wigglesworthia glossinidia [J].
Chen, XA ;
Li, S ;
Aksoy, S .
JOURNAL OF MOLECULAR EVOLUTION, 1999, 48 (01) :49-58
[3]   Tissue tropism, transmission and expression of foreign genes in vivo in midgut symbionts of tsetse flies [J].
Cheng, Q ;
Aksoy, S .
INSECT MOLECULAR BIOLOGY, 1999, 8 (01) :125-132
[4]   Polymorphic microsatellites in Simulium damnosum s.l. and their use for differentiating two savannah populations:: implications for epidemiological studies [J].
Dumas, V ;
Herder, S ;
Bebba, A ;
Cadoux-Barnabé, C ;
Bellec, C ;
Cuny, G .
GENOME, 1998, 41 (02) :154-161
[5]  
ESTOUP A, 1995, MOL BIOL EVOL, V12, P1074
[6]   Homoplasy for size at microsatellite loci in humans and chimpanzees [J].
Garza, JC ;
Freimer, NB .
GENOME RESEARCH, 1996, 6 (03) :211-217
[7]   GENETIC-BASIS OF STERILITY IN HYBRID MALES FROM CROSSES OF GLOSSINA-MORSITANS-MORSITANS AND GLOSSINA-MORSITANS-CENTRALIS (DIPTERA, GLOSSINIDAE) [J].
GOODING, RH .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1987, 65 (03) :640-646
[8]   GENETICS OF 2 COLONIES OF GLOSSINA-PALLIDIPES ORIGINATING FROM ALLOPATRIC POPULATIONS IN KENYA [J].
GOODING, RH ;
MOLOO, SK .
MEDICAL AND VETERINARY ENTOMOLOGY, 1994, 8 (02) :133-136
[10]   GENETIC-VARIATION IN A TANZANIAN POPULATION OF GLOSSINA-SWYNNERTONI (DIPTERA, GLOSSINIDAE) [J].
GOODING, RH ;
MBISE, S ;
MACHA, P ;
ROLSETH, BM .
JOURNAL OF MEDICAL ENTOMOLOGY, 1993, 30 (02) :489-492