Altered enteric microbiota ecology in interleukin 10-deficient mice during development and progression of intestinal inflammation

被引:178
作者
Maharshak, Nitsan [1 ,2 ]
Packey, Christopher D. [1 ,3 ]
Ellermann, Melissa [1 ,3 ]
Manick, Sayeed [1 ]
Siddle, Jennica P. [1 ]
Huh, Eun Young [1 ]
Plevy, Scott [1 ,3 ]
Sartor, R. Balfour [1 ,3 ]
Carroll, Ian M. [1 ]
机构
[1] Univ North Carolina Chapel Hill, Div Gastroenterol & Hepatol, Ctr Gastrointestinal Biol & Dis, Chapel Hill, NC 27599 USA
[2] Tel Aviv Univ, Dept Gastroenterol & Liver Dis, Tel Aviv Sourasky Med Ctr, Sackler Sch Med, Tel Aviv, Israel
[3] Univ North Carolina Chapel Hill, Dept Microbiol & Immunol, Chapel Hill, NC USA
关键词
inflammatory bowel diseases; intestinal microbiota; Interleukin 10-deficient mouse;
D O I
10.4161/gmic.25486
中图分类号
R57 [消化系及腹部疾病];
学科分类号
100201 [内科学];
摘要
Inflammatory bowel diseases (IBD) result from dysregulated immune responses toward microbial and perhaps other luminal antigens in a genetically susceptible host, and are associated with altered composition and diversity of the intestinal microbiota. The interleukin 10-deficient (IL-10(-/-)) mouse has been widely used to model human IBD; however the specific alterations that occur in the intestinal microbiota of this mouse model during the onset of colonic inflammation have not yet been defined. The aim of our study was to define the changes in diversity and composition that occur in the intestinal microbiota of IL-10(-/-) mice during the onset and progression of colonic inflammation. We used high throughput sequencing of the 16S rRNA gene to characterize the diversity and composition of formerly germ-free, wildtype and IL-10(-/-) mice associated with the same intestinal microbiota over time. Following two weeks of colonization with a specific pathogen-free (SPF) microbiota we observed a significant increase in the diversity and richness of the intestinal microbiota of wild-type mice. In contrast, a progressive decrease in diversity and richness was observed at three and four weeks in IL-10(-/-) mice. This decrease in diversity and richness was mirrored by an increase in Proteobacteria and Escherichia coli in IL-10(-/-) mice. An increase in E. coli was also observed in conventionally raised IL-10(-/-) mice at the point of colonic inflammation. Our data report the sequential changes in diversity and composition of the intestinal microbiota in an immune-mediated mouse model that may help provide insights into the primary vs. secondary role of dysbiosis in human IBD patients.
引用
收藏
页码:316 / 324
页数:9
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