Common architecture of nuclear receptor heterodimers on DNA direct repeat elements with different spacings

被引：140

作者：

Rochel, Natacha ^{[1
,2
,3
,4
]}

Ciesielski, Fabrice ^{[1
,2
,3
,4
]}

Godet, Julien ^{[4
,5
,6
]}

Moman, Edelmiro ^{[1
,2
,3
,4
]}

Roessle, Manfred ^{[7
]}

Peluso-Iltis, Carole ^{[1
,2
,3
,4
]}

Moulin, Martine ^{[8
]}

Haertlein, Michael ^{[8
]}

Callow, Phil ^{[8
]}

Mely, Yves ^{[4
,5
,6
]}

Svergun, Dmitri I. ^{[7
]}

Moras, Dino ^{[1
,2
,3
,4
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, Illkirch Graffenstaden, France

[2] INSERM, U964, Illkirch Graffenstaden, France

[3] CNRS, UMR 7104, Illkirch Graffenstaden, France

[4] Univ Strasbourg, Illkirch Graffenstaden, France

[5] Fac Pharm, Lab Biophoton & Pharmacol, Illkirch Graffenstaden, France

[6] CNRS, UMR 7213, Illkirch Graffenstaden, France

[7] DESY, European Mol Biol Lab, Hamburg Outstn, D-2000 Hamburg, Germany

[8] Inst Max Von Laue Paul Langevin, F-38042 Grenoble, France

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 05期

关键词：

VITAMIN-D-RECEPTOR; SMALL-ANGLE SCATTERING; RETINOID-X-RECEPTOR; LIGAND-BINDING DOMAIN; CRYSTAL-STRUCTURE; TRANSCRIPTIONAL-ACTIVATION; STRUCTURAL DETERMINANTS; RESPONSE ELEMENT; HORMONE-RECEPTOR; NATURAL LIGAND;

D O I：

10.1038/nsmb.2054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear hormone receptors (NHRs) control numerous physiological processes through the regulation of gene expression. The present study provides a structural basis for understanding the role of DNA in the spatial organization of NHR heterodimers in complexes with coactivators such as Med1 and SRC-1. We have used SAXS, SANS and FRET to determine the solution structures of three heterodimer NHR complexes (RXR-RAR, PPAR-RXR and RXR-VDR) coupled with the NHR interacting domains of coactivators bound to their cognate direct repeat elements. The structures show an extended asymmetric shape and point to the important role played by the hinge domains in establishing and maintaining the integrity of the structures. The results reveal two additional features: the conserved position of the ligand-binding domains at the 5' ends of the target DNAs and the binding of only one coactivator molecule per heterodimer, to RXR's partner.

引用

页码：564 / U207

页数：8

共 48 条

[1] Dynamics of coactivator recruitment and chromatin modifications during nuclear receptor mediated transcription [J].