Transmission of Rickettsia massiliae in the tick, Rhipicephalus turanicus

被引:100
作者
Matsumoto, K [1 ]
Ogawa, M [1 ]
Brouqui, P [1 ]
Raoult, D [1 ]
Parola, P [1 ]
机构
[1] CNRS, Unite Rickettsies, UMR 6020,IFR 48,Fac Med, WHO Collaborat Ctr Rickettsial Reference & Res, F-13385 Marseille, France
关键词
Rhipicephalus turanicus; Rickettsia massiliae Bar29; transmission by co-feeding; transovarial transmission; transstadial transmission; Corsica;
D O I
10.1111/j.1365-2915.2005.00569.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Rickettsia massiliae, strain Bar29, was detected in engorged female ticks of the Rhiphicephalus sanguineus group collected in Corsica, a French Mediterranean island. Ticks were identified by molecular analysis as Rhipicephalus turanicus (Pomerantsev) (Acari: Ixodidae). Twenty larvae of the second generation obtained from a R. massiliae-infected, engorged female were tested by polymerase chain reaction (PCR) and all were positive for R. massiliae. Larvae of the same cohort were fed on rabbits and specimens of subsequent stages of the second and third generation of ticks were tested by PCR. Both transovarial and transstadial transmission were demonstrated; the transovarial transmission rate was estimated at 100%. A high filial infection rate was demonstrated; 132 out of 134 larvae obtained from five infected females of the fourth generation were infected. When saliva samples from half-engorged Rh. turanicus of the second generation were tested by PCR, four out of five were positive. Rickettsia massiliae was detected in faeces of infected ticks by PCR and immunofluorescence assay, although no rickettsiae could be maintained in culture. Co-feeding/transsexual transmission of R. massiliae Bar29 was demonstrated by feeding male Rh. turanicus on a rabbit with Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) females (the latter were the only uninfected ticks available). Infection was subsequently detected in nine out of the thirteen females (69.2%). These results suggest that Rh. turanicus ticks are potential vectors and reservoirs for R. massiliae Bar29.
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页码:263 / 270
页数:8
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