Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth

被引：52

作者：

Cappai, R ^{[1
]}

Mok, SS

Galatis, D

Tucker, DF

Henry, A

Beyreuther, K

Small, DH

Masters, CL

机构：

[1] Univ Melbourne, Dept Pathol, Parkville, Vic 3052, Australia

[2] Mental Hlth Res Inst, Parkville, Vic 3052, Australia

[3] Univ Heidelberg, Ctr Mol Biol, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 1999年 / 442卷 / 01期

关键词：

amyloid precursor-like protein 2; neuritogenesis;

D O I：

10.1016/S0014-5793(98)01635-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins. (C) 1999 Federation of European Biochemical Societies.

引用

页码：95 / 98

页数：4

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