Physically Crosslinked Nanocomposites from Silicate-Crosslinked PEO: Mechanical Properties and Osteogenic Differentiation of Human Mesenchymal Stem Cells

被引：119

作者：

Gaharwar, Akhilesh K. ^{[1
]}

Kishore, Vipuil ^{[1
]}

Rivera, Christian ^{[1
]}

Bullock, Whitney ^{[1
]}

Wu, Chia-Jung ^{[1
]}

Akkus, Ozan ^{[1
]}

Schmidt, Gudrun ^{[1
]}

机构：

[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 06期

关键词：

human mesenchymal stem cells; mechanical properties; nanocomposites; poly(ethylene oxide); silicate nanoparticles; POLYMER-CLAY SOLUTIONS; ANGLE NEUTRON-SCATTERING; POLY(ETHYLENE OXIDE); FLOW-BIREFRINGENCE; IN-VITRO; HYDROGELS; LAPONITE; RHEOLOGY; DESIGN; BONE;

D O I：

10.1002/mabi.201100508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The mechanical and biological properties of silicate-crosslinked PEO nanocomposites are studied. A strong correlation is observed between silicate concentration and mechanical properties. In vitro cell culture studies reveal that an increase in silicate concentration enhances the attachment and proliferation of human mesenchymal stem cells significantly. An upregulation in the expression of osteocalcin on nanocomposites compared to the tissue culture polystyrene control is observed. Together, these results suggest that silicate-based nanocomposites are bioactive and have the potential to be used in a range of biotechnological and biomedical applications such as injectable matrices, biomedical coatings, drug delivery, and regenerative medicine.

引用

页码：779 / 793

页数：15

共 81 条

[1]

Mechanical properties of hydrogels and their experimental determination [J].

Anseth, KS ;

Bowman, CN ;

BrannonPeppas, L .

BIOMATERIALS, 1996, 17 (17) :1647-1657

[2]

Rheology and gelation kinetics in laponite dispersions containing poly(ethylene oxide) [J].

Baghdadi, HA ;

Sardinha, H ;

Bhatia, SR .

JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 2005, 43 (02) :233-240

[3]

Evidence for re-entrant behavior in laponite-PEO systems [J].

Baghdadi, Hossein A. ;

Jensen, Elizabeth C. ;

Easwar, Nalini ;

Bhatia, Surita R. .

RHEOLOGICA ACTA, 2008, 47 (02) :121-127

[4]

Engineering Substrate Topography at the Micro- and Nanoscale to Control Cell Function [J].

Bettinger, Christopher J. ;

Langer, Robert ;

Borenstein, Jeffrey T. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (30) :5406-5415

[5]

Bioinspired design and assembly of platelet reinforced polymer films [J].

Bonderer, Lorenz J. ;

Studart, Andre R. ;

Gauckler, Ludwig J. .

SCIENCE, 2008, 319 (5866) :1069-1073

[6]

Synergy between genetic and tissue engineering:: Runx2 overexpression and in vitro construct development enhance in vivo mineralization [J].

Byers, BA ;

Guldberg, RE ;

García, AJ .

TISSUE ENGINEERING, 2004, 10 (11-12) :1757-1766

[7]

SILICON . A POSSIBLE FACTOR IN BONE CALCIFICATION [J].

CARLISLE, EM .

SCIENCE, 1970, 167 (3916) :279-&

[8]

PEG/clay nanocomposite hydrogel: a mechanically robust tissue engineering scaffold [J].

Chang, Chien-Wen ;

van Spreeuwel, Ariane ;

Zhang, Chao ;

Varghese, Shyni .

SOFT MATTER, 2010, 6 (20) :5157-5164

[9]

Geometric control of cell life and death [J].

Chen, CS ;

Mrksich, M ;

Huang, S ;

Whitesides, GM ;

Ingber, DE .

SCIENCE, 1997, 276 (5317) :1425-1428

[10]

The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder [J].

Dalby, Matthew J. ;

Gadegaard, Nikolaj ;

Tare, Rahul ;

Andar, Abhay ;

Riehle, Mathis O. ;

Herzyk, Pawel ;

Wilkinson, Chris D. W. ;

Oreffo, Richard O. C. .

NATURE MATERIALS, 2007, 6 (12) :997-1003

← 1 2 3 4 5 6 7 8 9 →