Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures

被引：17

作者：

Adir, N ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Dept Chem, IL-32000 Haifa, Israel

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444998016497

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxygen-evolving photosystem II reaction centres (RCII) isolated from both spinach and pea have been crystallized. A single crystal form grew from RCII monomers in the presence of nine different three-component mixtures of non-ionic detergents and heptane-1,2,3-triol. The crystals grew as hexagonal rods with dimensions of up to 1 x 0.3 x 0.3 mm. The crystals diffracted to a maximum resolution of 6.5 Angstrom and belong to a hexagonal space group with unit-cell parameters a = 495, b = 495, c = 115 Angstrom, alpha = beta = 90, gamma = 120 degrees. The growth of a single crystal form in the presence of such a large Variety of detergents suggests a very limited range of crystal lattice formation sites in the RCII complex.

引用

页码：891 / 894

页数：4

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