Sulfa drug screening in yeast:: fifteen sulfa drugs compete with p-aminobenzoate in Saccharomyces cerevisiae

被引：26

作者：

Castelli, LA ^{[1
]}

Nguyen, NP ^{[1
]}

Macreadie, IG ^{[1
]}

机构：

[1] Biomol Res Inst, Parkville, Vic 3052, Australia

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 199卷 / 02期

关键词：

drug resistance; sulfone; sulfonamide; p-aminobenzoate synthase;

D O I：

10.1016/S0378-1097(01)00172-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sulfa drugs have been used as antimicrobials for decades but resistance is now a problem. For major eukaryotic pathogens, including Plasmodium and Pneumocystis. sulfa drug testing is difficult or impossible. We have shown that the eukaryote yeast can be used as a model fur the study of sulfa drugs within certain parameters. Fifteen sulfa drugs inhibited yeast growth in a manner indicating competition with p-aminobenzoate (pABA). Such competition resulted from direct addition of pABA or through increased expression of the pABA synthase gene (ABZI). The model system predicts that overexpression of the pABA synthase gene can lead to drug resistance. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：181 / 184

页数：4

共 5 条

[1]

Connor E. E., 1998, Primary Care Update for Ob/Gyns, V5, P32, DOI 10.1016/S1068-607X(97)00121-2

[2] PARA-AMINOBENZOATE SYNTHASE GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A BIFUNCTIONAL ENZYME [J].