Oxidation of ferulic acid by laccase: identification of the products and inhibitory effects of some dipeptides

被引：65

作者：

Carunchio, F

Crescenzi, C

Girelli, AM

Messina, A

Tarola, AM

机构：

[1] Univ Roma La Sapienza, Dipartimento Controllo & Gest Merci & Loro Impatt, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

TALANTA | 2001年 / 55卷 / 01期

关键词：

laccase; inhibitors; dipeptides; high performance liquid chromatography;

D O I：

10.1016/S0039-9140(01)00417-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The laccase activity, in the presence of ferulic acid as substrate, was tested with high performance liquid chromatography (HPLC). The two products initially formed were identified by HPLC-mass spectrometry and HPLC diode array as trans-5-[(E)-2-carboxyvinyl]-2-(4-hydroxy-3-methoxy-phenyl)-7-methoxy-2,3-dihydrobenzofuran-3-carboxylic acid and (Z)-beta-{4-[(E)-2-carboxyvinyl]-2-methoxyphenoxy}-4-hydroxy-3-methoxy cinnamic acid. The inhibitory effects of some dipeptides due to the formation of copper complexes have also been reported. The results obtained confirm that the inhibitory effects on laccase activity are strongly related to the structure of the dipeptides and confirm the important role of copper(II) in the reaction mechanism. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：189 / 200

页数：12

共 17 条

[1] SPECTROGRAPHIC CONTRIBUTIONS TO LIGNIN CHEMISTRY .9. ABSORPTION PROPERTIES OF SOME 4-HYDROXYPHENYL GUAIACYL AND 4-HYDROXY-3,5-DIMETHOXYPHENYL TYPE MODEL COMPOUNDS FOR HARDWOOD LIGNINS [J].