Effects of Platelet-Rich Plasma on the Quality of Repair of Mechanically Induced Core Lesions in Equine Superficial Digital Flexor Tendons: A Placebo-Controlled Experimental Study

被引:203
作者
Bosch, Gerco [1 ]
van Schie, Hans T. A. [1 ,2 ]
de Groot, Mark W. [3 ]
Cadby, Jennifer A. [1 ,2 ]
van de Lest, Chris H. A. [1 ,4 ]
Barneveld, Ab [1 ]
van Weeren, P. Rene [1 ]
机构
[1] Univ Utrecht, Dept Equine Sci, Fac Vet Med, NL-3584 CM Utrecht, Netherlands
[2] Erasmus MC Univ Med Ctr, Dept Orthopaed, Rotterdam, Netherlands
[3] Univ Utrecht, Dept Pathobiol, Fac Vet Med, Utrecht, Netherlands
[4] Univ Utrecht, Fac Vet Med, Dept Biochem & Cell Biol, Utrecht, Netherlands
关键词
platelet-rich plasma (PRP); tendon; collagen; histology; repair; GROWTH-FACTOR-I; GENE-EXPRESSION PATTERNS; ACHILLES-TENDON; TENDINITIS; COLLAGEN; VITRO; PROLIFERATION; PATHOGENESIS; INJECTION; HORSES;
D O I
10.1002/jor.20980
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
100224 [整形外科学];
摘要
Tendon injuries are notorious for their slow and functionally inferior healing. Intratendinous application of platelet-rich plasma (PRP) has been reported to stimulate the repair process of tendon injuries, but there is little conclusive evidence for its effectiveness. A placebo-controlled experimental trial was performed to test the hypothesis that a single intratendinous PRP treatment enhances the quality of tendon repair, as evidenced by improved biochemical, biomechanical, and histological tissue properties. In six horses, tendon lesions were created surgically in the Superficial Digital Flexor Tendons (SDFT) of both front limbs, one of which was treated with PRP and the other with saline. After 24 weeks, the tendons were harvested for biochemical, biomechanical, and histological evaluations. Collagen, glycosaminoglycan, and DNA content (cellularity) was higher in PRP-treated tendons (p = 0.039, 0.038, and 0.034, respectively). The repair tissue in the PRP group showed a higher strength at failure (p = 0.021) and Elastic Modulus (p = 0.019). Histologically, PRP-treated tendons featured better organization of the collagen network (p = 0.031) and signs of increased metabolic activity (p = 0.031). It was concluded that PRP increases metabolic activity and seems to advance maturation of repair tissue over nontreated experimentally induced tendon lesions, which suggests that PRP might be beneficial in the treatment of clinical tendon injuries. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:211-217, 2010
引用
收藏
页码:211 / 217
页数:7
相关论文
共 44 条
[1]
Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture [J].
Anitua, E ;
Andía, I ;
Sanchez, M ;
Azofra, J ;
Zalduendo, MD ;
de la Fuente, M ;
Nurden, P ;
Nurden, AT .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2005, 23 (02) :281-286
[2]
Autologous platelet concentrates as a treatment for musculoskeletal lesions in five horses [J].
Arguelles, D. ;
Carmona, J. U. ;
Climent, F. ;
Munoz, E. ;
Prades, M. .
VETERINARY RECORD, 2008, 162 (07) :208-211
[3]
Platelet concentrate injection improves Achilles tendon repair in rats [J].
Aspenberg, P ;
Virchenko, O .
ACTA ORTHOPAEDICA SCANDINAVICA, 2004, 75 (01) :93-99
[4]
ASTROM M, 1995, CLIN ORTHOP RELAT R, P151
[5]
Are the material properties and matrix composition of equine flexor and extensor tendons determined by their functions? [J].
Batson, EL ;
Paramour, RJ ;
Smith, TJ ;
Birch, HL ;
Patterson-Kane, JC ;
Goodship, AE .
EQUINE VETERINARY JOURNAL, 2003, 35 (03) :314-318
[6]
Platelet-rich plasma - Clinical applications in dentistry [J].
Carlson, NE ;
Roach, RB .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 2002, 133 (10) :1383-1386
[7]
Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry [J].
Colgrave, Michelle L. ;
Allingham, Peter G. ;
Jones, Alun .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1212 (1-2) :150-153
[8]
Crevier N, 1996, AM J VET RES, V57, P1111
[9]
IMMATURE TENDON ADAPTATION TO STRENUOUS EXERCISE [J].
CURWIN, SL ;
VAILAS, AC ;
WOOD, J .
JOURNAL OF APPLIED PHYSIOLOGY, 1988, 65 (05) :2297-2301
[10]
Insulin-like growth factor-I improves cellular and molecular aspects of healing in a collagenase-induced model of flexor tendinitis [J].
Dahlgren, LA ;
van der Meulen, MCH ;
Bertram, JEA ;
Starrak, GS ;
Nixon, AJ .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2002, 20 (05) :910-919