Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration

被引:76
作者
Lee, JS
Ray, R
Chien, CB
机构
[1] Univ Utah, Med Ctr, Dept Neurobiol & Anat, MREB 401, Salt Lake City, UT 84132 USA
[2] Univ Utah, Program Mol Biol, Salt Lake City, UT USA
关键词
zebrafish; Roundabout; Slit; cell migration; axon guidance;
D O I
10.1002/dvdy.1136
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
We report the cloning and expression patterns of three novel zebrafish Roundabout homologs. The Roundabout (robo) gene encodes a transmembrane receptor that is essential for axon guidance in Drosophila and Robe family members have been implicated in cell migration. Analysis of extracellular domains and conserved cytoplasmic motifs shows that zebrafish Robo1 and Robo2 are orthologs of mammalian Robo1 and Robo2, respectively, while zebrafish Robo3 is likely to be an ortholog of mouse Rig-1. The three zebrafish robes are expressed in distinct but overlapping patterns during embryogenesis. They are highly expressed in the developing nervous system, including the olfactory system, visual system, hindbrain, cranial ganglia, spinal cord, and posterior lateral line primordium. They are also expressed in several nonneuronal tissues, including somites and fin buds. The timing and patterns of expression suggest roles for zebrafish robes in axon guidance and cell migration.
引用
收藏
页码:216 / 230
页数:15
相关论文
共 73 条
[1]   Slit2-mediated chemorepulsion and collapse of developing forebrain axons [J].
Ba-Charvet, KTN ;
Brose, K ;
Marillat, V ;
Kidd, T ;
Goodman, CS ;
Tessier-Lavigne, M ;
Sotelo, C ;
Chédotal, A .
NEURON, 1999, 22 (03) :463-473
[2]   Repulsive axon guidance: Abelson and enabled play opposing roles downstream of the roundabout receptor [J].
Bashaw, GJ ;
Kidd, T ;
Murray, D ;
Pawson, T ;
Goodman, CS .
CELL, 2000, 101 (07) :703-715
[3]   Chimeric axon guidance receptors: The cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion [J].
Bashaw, GJ ;
Goodman, CS .
CELL, 1999, 97 (07) :917-926
[4]  
Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]
[5]   IDENTIFICATION OF SPINAL NEURONS IN THE EMBRYONIC AND LARVAL ZEBRAFISH [J].
BERNHARDT, RR ;
CHITNIS, AB ;
LINDAMER, L ;
KUWADA, JY .
JOURNAL OF COMPARATIVE NEUROLOGY, 1990, 302 (03) :603-616
[6]  
Bernhardt RR, 1999, J NEUROBIOL, V38, P137, DOI 10.1002/(SICI)1097-4695(199901)38:1<137::AID-NEU11>3.0.CO
[7]  
2-P
[8]  
Brand M, 1996, DEVELOPMENT, V123, P179
[9]   Mechanisms controlling sex myoblast migration in Caenorhabditis elegans hermaphrodites [J].
Branda, CS ;
Stern, MJ .
DEVELOPMENTAL BIOLOGY, 2000, 226 (01) :137-151
[10]   Slit proteins bind robe receptors and have an evolutionarily conserved role in repulsive axon guidance [J].
Brose, K ;
Bland, KS ;
Wang, KH ;
Arnott, D ;
Henzel, W ;
Goodman, CS ;
Tessier-Lavigne, M ;
Kidd, T .
CELL, 1999, 96 (06) :795-806