Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages

被引：69

作者：

Bae, Deok Sung ^{[1
]}

Kim, Young Hoon ^{[1
]}

Pan, Cheol-Ho ^{[2
]}

Nho, Chu Won ^{[2
]}

Samdan, Javzan ^{[3
,4
]}

Yansan, Jamyansan ^{[3
,4
]}

Lee, Jae Kwon ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Educ, Dept Biol Educ, Cheongju 361763, South Korea

[2] Korea Inst Sci & Technol, Funct Food Ctr, Kangnung 210340, South Korea

[3] MAS, Inst Chem, Ulaanbaatar 51, Mongolia

[4] MAS, Inst Chem Technol, Ulaanbaatar 51, Mongolia

来源：

BMB REPORTS | 2012年 / 45卷 / 02期

关键词：

Cyclooxygenase-2; Cytokine; Inflammation; Macrophage; Nitric oxide; Protopine; NITRIC-OXIDE; NUCLEAR-FACTOR; PHORBOL ESTER; KAPPA-B; EXPRESSION; CYTOKINES; INTERLEUKIN-1; INHIBITION; ALPHA; GENE;

D O I：

10.5483/BMBRep.2012.45.2.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin E-2 (PGE(2)) production by U'S-stimulated Raw 264.7 cells, without a cytotoxic effect. Pm-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B). [BMB reports 2012; 45(2): 108-113]

引用

页码：108 / 113

页数：6

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