Retinal overexpression of angiopoietin-2 mimics diabetic retinopathy and enhances vascular damages in hyperglycemia

被引：85

作者：

Pfister, Frederick ^{[1
]}

Wang, Yumei ^{[1
]}

Schreiter, Kay ^{[2
]}

vom Hagen, Franziska ^{[1
]}

Altvater, Karin ^{[1
]}

Hoffmann, Sigrid ^{[3
]}

Deutsch, Urban ^{[4
]}

Hammes, Hans-Peter ^{[1
]}

Feng, Yuxi ^{[1
]}

机构：

[1] Univ Heidelberg, Med Fac Mannheim, Med Clin 5, D-68167 Mannheim, Germany

[2] DeveloGen AG, D-37079 Gottingen, Germany

[3] Univ Heidelberg, Med Fac Mannheim, Med Res Ctr, D-68167 Mannheim, Germany

[4] Univ Bern, Theodor Kocher Inst, CH-3012 Bern, Switzerland

来源：

ACTA DIABETOLOGICA | 2010年 / 47卷 / 01期

关键词：

Angiopoietin-2; Diabetic retinopathy; Pericyte; GROWTH-FACTOR; MULLER CELLS; ANGIOGENESIS; EXPRESSION;

D O I：

10.1007/s00592-009-0099-2

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Our previous data suggested that angiopoietin-2 (Ang-2) is linked to pericyte loss, thereby playing an important role in diabetic retinopathy. In this study, we investigated the effect of retinal overexpression of human Ang-2 (mOpsinhAng2 mouse) on vascular morphology in non-diabetic and streptozotozin-induced diabetic animals. Pericyte (PC) coverage and acellular capillary (AC) formation were quantitated in retinal digest preparations after 3 and 6 months of diabetes duration. The degree of retinopathy in non-diabetic mOpsinhAng2 mice at 3 months (-21% PC, +49% AC) was comparable to age-matched diabetic wild type mice. Diabetic mOpsinhAng2 mice exhibited significantly worse vascular pathology than wild type counterparts at 6 months. Quantitative PCR revealed that human Ang-2 mRNA was highly overexpressed in retinas of transgenic mice. Our data demonstrate that overexpression of Ang-2 in the retina enhances vascular pathology, indicating that Ang-2 plays an essential role in diabetic vasoregression via destabilization of pericytes.

引用

页码：59 / 64

页数：6

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