The inhibitory effect of Gynostemma pentaphyllum on MCP-1 and type I procollagen expression in rat hepatic stellate cells

被引：43

作者：

Chen, Ming-Ho ^{[1
,2
,3
]}

Wang, Qwa-Fun ^{[1
,3
]}

Chen, Lih-Geeng ^{[1
]}

Shee, Jia-Jen ^{[4
]}

Chen, Jung-Chou ^{[3
]}

Chen, Ke-Yu ^{[1
]}

Chen, Shu-Hsin ^{[1
]}

Su, Jyan-Gwo J. ^{[5
]}

Liu, Yi-Wen ^{[1
]}

机构：

[1] Natl Chiayi Univ, Coll Life Sci, Grad Inst Biomed & Biopharmaceut Sci, Chiayi 600, Taiwan

[2] Chiayi Christian Hosp, Dept Chinese Med, Chiayi, Taiwan

[3] Chinese Med Univ, Sch Post Baccalaureate Chinese Med, Taichung, Taiwan

[4] Chang Gung Mem Hosp, Dept Surg, Chiayi, Taiwan

[5] Natl Chiayi Univ, Coll Life Sci, Dept Biochem Sci & Technol, Chiayi 600, Taiwan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2009年 / 126卷 / 01期

关键词：

Gynostemma pentaphyllum; Gypenosides; Monocyte chemoattractant protein-1; Rat hepatic stellate cells; Type I procollagen; Anti-proliferation; ANOECTOCHILUS-FORMOSANUS; DAMMARANE SAPONINS; LIVER-INJURY; ACTIVATION; GYPENOSIDE; APOPTOSIS; FIBROSIS; CHROMATOGRAPHY; MEDICINE; MAKINO;

D O I：

10.1016/j.jep.2009.08.012

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Aim of the study: Gynostemma pentaphyllum is a popular folk medicine that has been used for treatment of hepatitis in Asia. Our previous study demonstrates that Gynostemma pentaphyllum n-butanol extract inhibits the onset and improves the recovery of CCl4-induced liver fibrogenesis in rats and inhibits PDGF-induced rat hepatic stellate cells (HSCs) proliferation. In this study. the effect of Gynostemma pentaphyllum extract oil cytokines and type I procollagen expression was analyzed. Materials and methods: Rat HSCs were treated with PDGF, Gynostemma pentaphyllum n-butanol extract, RP-18-Gyp fraction, rapamycin or vehicle. Rat cytokine antibody array chip or ELISA kit was used for cytokines detection. Intracellular protein expression was detected by Western blotting. mRNA expression was analyzed by RT-PCR. Results: RP-18-Gyp fraction is the more purified gypenosides fraction from Gynostemma pentaphyllum n-butanol extract. in cell proliferation, the inhibitory effect of 200 mu g/ml RP-18-Cyp fraction is similar to 500 mu g/ml Gynostemma pentaphyllum n-butanol extract. Furthermore, both of them have the ability of decreasing monocyte chemoattractant protein-1 (MCP-1) mRNA expression and protein release and inhibiting type I procollagen protein expression. Conclusions: Both of Gynostemma pentaphyllum n-butanol extract and its more purified RP-18-Gyp fraction have the biological activities in the inhibition of cell proliferation, MCP-1 release and type I procollagen expression in rat HSCs. These data could provide the evidence to support for the traditional use of Gynostemma pentaphyllum in hepatitis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：42 / 49

页数：8

共 30 条

[1]

Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-κB-mediated iNOS protein expression [J].