Interactions of tea tannins and condensed tannins with proteins

被引：244

作者：

Frazier, Richard A. ^{[1
]}

Deaville, Eddie R. ^{[2
]}

Green, Rebecca J. ^{[3
]}

Stringano, Elisabetta ^{[2
]}

Willoughby, Ian ^{[4
]}

Plant, John ^{[5
]}

Mueller-Harvey, Irene ^{[2
]}

机构：

[1] Univ Reading, Dept Food Biosci, Reading RG6 6AP, Berks, England

[2] Univ Reading, Dept Agr, Anim Sci Res Grp, Chem & Biochem Lab, Reading RG6 6AT, Berks, England

[3] Univ Reading, Reading Sch Pharm, Reading RG6 6AP, Berks, England

[4] Varian Associates Inc, Church Stretton SY6 6AX, Shrops, England

[5] Univ Reading, Dept Chem, Reading RG6 6AD, Berks, England

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 51卷 / 02期

关键词：

Condensed tannin; Polyphenol; Protein; Albumin; Gelatin; Isothermal titration calorimetry; MASS-SPECTROMETRY; POLYMERIC PROCYANIDINS; PROANTHOCYANIDINS; POLYPHENOLS; HEALTH; CHROMATOGRAPHY; MECHANISMS; BINDING; HUMANS; SEEDS;

D O I：

10.1016/j.jpba.2009.05.035

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Binding parameters for the interactions of four types of tannins: tea catechins, grape seed proanthocyanidins, mimosa 5-deoxy proanthocyanidins,and sorghum procyanidins (mDP=17), with gelatin and bovine serum albumin (BSA) have been determined from isothermal titration calorimetry data. Equilibrium binding constants determined for the interaction with gelatin were in the range 10(4) to 10(6) M-1 and in the order: sorghum procyanidins > grape seed proanthocyanidins > mimosa 5-deoxy proanthocyanidins > tea catechins. Interaction with BSA was generally weaker, with equilibrium binding constants of <= 10(3) M-1 for grape seed proanthocyanidins, mimosa 5-deoxy proanthocyanidins and tea catechins, and 10(4) M-1 for the sorghum procyanidins. In all cases the interactions with proteins were exothermic and involved multiple binding sites on the protein. The data are discussed in relation to the structures and the known nutritional effects of the condensed tannins. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：490 / 495

页数：6

共 29 条

[1]

INTERACTIONS OF CONDENSED TANNINS WITH SELECTED PROTEINS [J].