Transplanted Bone Morphogenetic Protein/Poly(lactic-co-glycolic Acid) Delayed-release Microcysts Combined with Rat Micromorselized Bone and Collagen for Bone Tissue Engineering

被引：5

作者：

Ji, Y. ^{[1
]}

Xu, G. P. ^{[1
]}

Yan, J. L. ^{[1
]}

Pan, S. H. ^{[2
]}

机构：

[1] Harbin Med Univ, Dept Orthopaed Surg, Affiliated Hosp 1, Harbin 150001, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Cent Lab, Affiliated Hosp 1, Harbin 150001, Heilongjiang, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2009年 / 37卷 / 04期

基金：

中国国家自然科学基金;

关键词：

BONE TRANSPLANTATION; BONE TISSUE ENGINEERING; BONE MORPHOGENETIC PROTEIN 2; GROWTH FACTORS; CONTROLLED-RELEASE TECHNOLOGY; MICROCYST; MORSELIZED ALLOGRAFT; IN-VITRO; MICROSPHERES; PROTEIN; DELIVERY; DEFECTS; FUSION;

D O I：

10.1177/147323000903700412

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

This study was designed to optimize the preparation of delayed-release microcysts containing bone morphogenetic protein 2 (BMP-2) combined with poly(lactic-co-glycolic acid) (PLGA) and to investigate their osteogenic properties when combined with rat autologous micromorselized bone and collagen. Rat autologous micromorselized bone, collagen and BMP-2/PLGA delayed-release microcysts were implanted in various combinations into the rat gluteus maximus muscle sack model. The following post-operative measurements were made: general observations of the implant site, histological observations, osteogenesis measurements and alkaline phosphatase activity. Autologous micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts demonstrated significantly superior osteogenic properties than any of the other combinations of these three components. These findings suggest that micromorselized bone combined with collagen and BMP-2/PLGA delayed-release microcysts could reduce the quantity of BMP-2 and autologous bone required for these procedures, making their use feasible in human bone restoration.

引用

页码：1075 / 1087

页数：13

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