Effects of Berberine on Expression of LOX-1 and SR-BI in Human Macrophage-Derived Foam Cells Induced by ox-LDL

被引：54

作者：

Guan, Siming ^{[1
]}

Wang, Bin ^{[1
]}

Li, Wei ^{[1
]}

Guan, Jinghuan ^{[2
]}

Fang, Xin ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Geriatr, Wuhan 430022, Peoples R China

[2] Hosp Combining Tradit Chinese Med & Western Med W, Wuhan 430024, Peoples R China

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2010年 / 38卷 / 06期

关键词：

Berberine; Foam Cell; Lectin-Like ox-LDL Receptor-1; Scavenger Receptor Class B Type I; LOW-DENSITY-LIPOPROTEIN; SCAVENGER RECEPTOR; IN-VITRO; CHOLESTEROL; MECHANISM; PROMOTES; STATINS; PATHWAY; RABBITS; KINASE;

D O I：

10.1142/S0192415X10008548

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

This study investigates the effects of beriberine on the expression of lectin-like ox-LDL receptor-1 (LOX-1), scavenger receptor A (SR-A), SR class B type I (SR-BI) and ATP-binding cassette transporter A1 (ABCA1) in human macrophage-derived foam cells induced by ox-LDL. Different concentrations of Berberine were co-cultured with THP-1 derived foam cells. The mRNA and protein expressions of LOX-1, SR-A, SR-BI and ABCA1 were determined by RT-PCR and Western blot analysis, respectively. Ox-LDL significantly increased the expression of LOX-1 and inhibited the expression of SR-BI in a dose-and time-dependent manner. Berberine significantly inhibited the effects of ox-LDL in a dose-and time-dependent manner. Moreover, ox-LDL significantly promoted ABCA1 expression. However, berberine had no effect on SR-A or ABCA1 expression. Berberine can inhibit the expression of LOX-1 and promote the expression of SR-BI in macrophage-derived foam cells. Therefore, berberine could be used to treat atherosclerotic diseases.

引用

页码：1161 / 1169

页数：9

共 23 条

[1]

Extracellular signal-regulated kinase-dependent stabilization of hepatic low-density lipoprotein receptor mRNA by herbal medicine berberine [J].