Icariin, a natural flavonol glycoside, induces apoptosis in human hepatoma SMMC-7721 cells via a ROS/JNK-dependent mitochondrial pathway

被引：232

作者：

Li, Songgang ^{[1
]}

Dong, Ping ^{[1
]}

Wang, Jianwei ^{[2
]}

Zhang, Jie ^{[3
]}

Gu, Jun ^{[1
]}

Wu, Xiangsong ^{[4
]}

Wu, Wenguang ^{[4
]}

Fei, Xiaozhou ^{[4
]}

Zhang, Zhiping ^{[4
]}

Wang, Yong ^{[4
]}

Quan, Zhiwei ^{[1
]}

Liu, Yingbin ^{[1
,4
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai 200092, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Surg Oncol, Hangzhou 310009, Zhejiang, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Chongming Branch,Dept Gen Surg, Shanghai 202150, Chongming Cty, Peoples R China

[4] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Gen Surg, Hangzhou 310009, Zhejiang, Peoples R China

来源：

CANCER LETTERS | 2010年 / 298卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Hepatoma; Icariin; SMMC-7721; cells; Apoptosis; Reactive oxygen species; c-Jun N terminal kinase; EMBRYONIC STEM-CELLS; PROSTATE-CANCER CELLS; NF-KAPPA-B; IN-VITRO; HEPATOCELLULAR-CARCINOMA; CARDIOMYOCYTE DIFFERENTIATION; MECHANISM; OXYGEN; ACTIVATION; EXPRESSION;

D O I：

10.1016/j.canlet.2010.07.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

In this study the anticancer effect of icariin a natural flavonol glycoside against human hepatoma SMMC-7721 cells and the underlying mechanisms were investigated Icariin triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 ratio loss of mitochodrial membrane potential cytochrome c release and caspase cascade Moreover icariin induced a sustained activation of the phosphorylation of c-Jun N-terminal kinase (INK) but not p38 and ERK1/2 and SP600125 (an inhibitor of JNK) almost reversed icariin-induced apoptosis in SMMC-7721 cells In addition icariin provoked the generation of reactive oxygen species (ROS) in SMMC-7721 cells while the antioxidant N-acetyl cysteine almost completely blocked icariin-induced JNK activation and apoptosis Taken together these findings suggest that icariin induces apoptosis through a ROS/JNK-dependent mitochondrial pathway (C) 2010 Elsevier Ireland Ltd All rights reserved

引用

页码：222 / 230

页数：9

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