DNA barcoding of 18 species of Bovidae

被引：26

作者：

Cai YanSen ^{[2
,3
]}

Zhang Liang ^{[1
]}

Shen FuJun ^{[1
]}

Zhang WenPing ^{[1
]}

Hou Rong ^{[1
]}

Yue BiSong ^{[3
]}

Li Jing ^{[3
]}

Zhang ZhiHe ^{[1
]}

机构：

[1] Chengdu Res Base Giant Panda Breeding, Key Lab Conservat Biol Endangered Wildlife Sichua, Chengdu 610000, Peoples R China

[2] Luzhou Med Coll, Dept Med Biol & Genet, Luzhou 646000, Peoples R China

[3] Sichuan Univ, Key Lab Conservat Biol Endangered Wildlife Sichua, Coll Life Sci, Chengdu 610000, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2011年 / 56卷 / 02期

关键词：

DNA barcode; COI; Bovidae; conservation; endangered species; RATES; AMPLIFICATION; PRIMERS; INOSINE;

D O I：

10.1007/s11434-010-4302-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genetic divergences of mitochondrial cytochrome c oxidase subunit I genes, known as DNA barcodes, have been used in species identification in the animal kingdom. Barcodes can assist field workers and taxonomists to determine groups in need of taxa analysis, and facilitate the recognition of appropriate populations and scales for conservation planning. In this study, 18 species of Bovidae were selected to evaluate the effectiveness of DNA barcoding for species differentiation. The results showed that all but 2 species had unique DNA barcodes. The mean intraspecific variation was 0.63%, yielding a threshold of 6.3% for flagging putative species. The results supported the inference that barcode variation within species of mammals is somewhat higher than within other animal groups. The present study validated the effectiveness of barcoding for the identification of bovid species.

引用

页码：164 / 168

页数：5

共 33 条

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