In vitro generation of CD4+CD25+regulatory cells from murine naive T cells

被引：129

作者：

Fantini, Massimo C. ^{[2
]}

Dominitzki, Sabine ^{[1
]}

Rizzo, Angelo ^{[2
]}

Neurath, Markus F. ^{[1
]}

Becker, Christoph ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Immunol Lab, I Med Clin, D-55131 Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Immunol Lab, I Med Clin, D-55131 Mainz, Germany

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 07期

关键词：

D O I：

10.1038/nprot.2007.258

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

CD4+CD25+ regulatory T cells (Tregs) are crucial for the maintenance of immunological tolerance. Recent data indicate that Tregs not only develop in the thymus during ontogeny but can also differentiate from naive T cells in the periphery. The following protocol describes a method by which Tregs are generated in vitro by stimulation of naive T cells in the presence of transforming growth factor beta (Ti-Tregs). In vitro-induced regulatory T cells express markers of conventional Treg such as CD25 and the genetic program committing transcription factor FoxP3. Functionally the in vitro-generated Ti-Tregs suppress T-cell activation and proliferation while in vivo these cells have been proven to control inflammation in different animal models, suggesting a potential use of these cells for immunotherapy. The protocol can be completed within 5 days.

引用

页码：1789 / 1794

页数：6

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