A novel strategy for unambiguous DPB1 typing

被引：4

作者：

Varney, MD ^{[1
]}

Tait, BD ^{[1
]}

机构：

[1] Royal Melbourne Hosp, Dept Pathol, Tissue Typing Lab, Parkville, Vic 3050, Australia

来源：

EUROPEAN JOURNAL OF IMMUNOGENETICS | 1998年 / 25卷 / 05期

关键词：

D O I：

10.1046/j.1365-2370.1998.00115.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8-9 and 84-87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.

引用

页码：371 / 374

页数：4

共 10 条

[1]

[Anonymous], ESPRIT CACID

[2] Nomenclature for factors of the HLA system, 1996 [J].

Bodmer, JG ;

Marsh, SGE ;

Albert, ED ;

Bodmer, WF ;

Bontrop, RE ;

Charron, D ;

Dupont, B ;

Erlich, HA ;

Fauchet, R ;

Mach, B ;

Mayr, WR ;

Parham, P ;

Sasazuki, T ;

Schreuder, GMT ;

Strominger, JL ;

Svejgaard, A ;

Terasaki, PI .

TISSUE ANTIGENS, 1997, 49 (03) :297-321

[3] DNA TYPING FOR CLASS-II HLA ANTIGENS WITH ALLELE-SPECIFIC OR GROUP-SPECIFIC AMPLIFICATION .3. TYPING FOR 24 ALLELES OF HLA-DP [J].

FERNANDEZVINA, M ;

MORAES, ME ;

STASTNY, P .

HUMAN IMMUNOLOGY, 1991, 30 (01) :60-68

[4] COMPLETE NUCLEOTIDE-SEQUENCE OF A FUNCTIONAL HLA-DP-BETA GENE AND THE REGION BETWEEN THE DP-BETA-1 AND DP-ALPHA-1 GENES - COMPARISON OF THE 5' ENDS OF HLA CLASS-II GENES [J].

KELLY, A ;

TROWSDALE, J .

NUCLEIC ACIDS RESEARCH, 1985, 13 (05) :1607-1621

[5]

Kimura A, 1992, HLA 1991, V1, P397

[6] Six new DPB1 alleles identified in a study of 1,302 unrelated bone marrow donor-recipient pairs [J].

Noreen, H ;

Steiner, L ;

Davidson, M ;

Johnson, S ;

Segall, M ;

Begovich, AB .

TISSUE ANTIGENS, 1997, 49 (05) :512-516

[7] HLA-DR TYPING BY PCR AMPLIFICATION WITH SEQUENCE-SPECIFIC PRIMERS (PCR-SSP) IN 2 HOURS - AN ALTERNATIVE TO SEROLOGICAL DR TYPING IN CLINICAL-PRACTICE INCLUDING DONOR-RECIPIENT MATCHING IN CADAVERIC TRANSPLANTATION [J].

OLERUP, O ;

ZETTERQUIST, H .

TISSUE ANTIGENS, 1992, 39 (05) :225-235

[8] HLA-B27 TYPING BY A GROUP-SPECIFIC PCR AMPLIFICATION [J].

OLERUP, O .

TISSUE ANTIGENS, 1994, 43 (04) :253-256

[9]

SCHMITTGER I, 1996, IMMUNOGENETICS, V44, P405

[10] Three new DPB1 alleles identified in a Bantu-speaking population from central Cameroon [J].

Zimmerman, PA ;

Steiner, LL ;

Titanji, VPK ;

Nde, PN ;

Bradley, JE ;

Pogonka, T ;

Begovich, AB .

TISSUE ANTIGENS, 1996, 47 (04) :293-299

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