Expression of imprinted genes surrounding the callipyge mutation in ovine skeletal muscle

被引:14
作者
Vuocolo, T
Cockett, NE
Tellam, RL
机构
[1] CSIRO Livestock Ind, Queensland Biosci Precinct, St Lucia, Qld 4067, Australia
[2] Utah State Univ, Logan, UT 84322 USA
关键词
Dlk-1; GTL2; PEG11; MEG8;
D O I
10.1071/EA05049
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The callipyge mutation in sheep results in postnatal hypertrophy and leanness of skeletal muscles in the pelvic limbs and loins. Associated changes also occur in the expression of a number of imprinted genes flanking the site of the mutation, which lies at the telomeric end of ovine chromosome 18. The transcripts from several of these genes are either spliced or undergo substantial RNA processing, sometimes in a very complex manner. The current investigation examined the effects of the callipyge mutation on the relative expression of some of these splice variants in samples taken: at birth, when the muscle hypertrophy phenotype is not expressed; and at 12 weeks of age, when the phenotype is fully apparent. It was concluded that changes in the postnatal developmental expression pattern of Dlk-1 are closely associated with the expression of the phenotype and that the callipyge mutation may promote a fetal-like gene expression program for some genes during postnatal life.
引用
收藏
页码:879 / 892
页数:14
相关论文
共 63 条
[1]   Differential expression of the GTL2 gene within the callipyge region of ovine chromosome 18 [J].
Bidwell, CA ;
Shay, TL ;
Georges, M ;
Beever, JE ;
Berghmans, S ;
Cockett, NE .
ANIMAL GENETICS, 2001, 32 (05) :248-256
[2]   Expression of PEG11 and PEG11AS transcripts in normal and callipyge sheep [J].
Bidwell, Christopher A. ;
Kramer, Lauren N. ;
Perkins, Allison C. ;
Hadfield, Tracy S. ;
Moody, Diane E. ;
Cockett, Noelle E. .
BMC BIOLOGY, 2004, 2 (1)
[3]   Identification of tandemly-repeated C/D snoRNA genes at the imprinted human 14q32 domain reminiscent of those at the Prader-Willi/Angelman syndrome region [J].
Cavaillé, J ;
Seitz, H ;
Paulsen, M ;
Ferguson-Smith, AC ;
Bachellerie, JP .
HUMAN MOLECULAR GENETICS, 2002, 11 (13) :1527-1538
[4]   The callipyge mutation enhances the expression of coregulated imprinted genes in cis without affecting their imprinting status [J].
Charlier, C ;
Segers, K ;
Karim, L ;
Shay, T ;
Gyapay, G ;
Cockett, N ;
Georges, M .
NATURE GENETICS, 2001, 27 (04) :367-369
[5]   Human-ovine comparative sequencing of a 250-kb imprinted domain encompassing the callipyge (clpg) locus and identification of six imprinted transcripts:: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8 [J].
Charlier, C ;
Segers, K ;
Wagenaar, D ;
Karim, L ;
Berghams, S ;
Jaillon, O ;
Shay, T ;
Weissenbach, J ;
Cockett, N ;
Gyapay, G ;
Georges, M .
GENOME RESEARCH, 2001, 11 (05) :850-862
[6]   The callipyge mutation and other genes that affect muscle hypertrophy in sheep [J].
Cockett, NE ;
Smit, MA ;
Bidwell, CA ;
Segers, K ;
Hadfield, TL ;
Snowder, GD ;
Georges, M ;
Charlier, C .
GENETICS SELECTION EVOLUTION, 2005, 37 :S65-S81
[7]   Polar overdominance at the Ovine callipyge locus [J].
Cockett, NE ;
Jackson, SP ;
Shay, TL ;
Farnir, F ;
Berghmans, S ;
Snowder, GD ;
Nielsen, DM ;
Georges, M .
SCIENCE, 1996, 273 (5272) :236-238
[8]   Analysis of the sheep genome [J].
Cockett, NE ;
Shay, TL ;
Smit, M .
PHYSIOLOGICAL GENOMICS, 2001, 7 (02) :69-78
[9]   CHROMOSOMAL LOCALIZATION OF THE CALLIPYGE GENE IN SHEEP (OVIS-ARIES) USING BOVINE DNA MARKERS [J].
COCKETT, NE ;
JACKSON, SP ;
SHAY, TL ;
NIELSEN, D ;
MOORE, SS ;
STEELE, MR ;
BARENDSE, W ;
GREEN, RD ;
GEORGES, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3019-3023
[10]   Alternative splicing and imprinting control of the Meg3/Gtl2-Dlk1 locus in mouse embryos [J].
Croteau, S ;
Charron, MC ;
Latham, KE ;
Naumova, AK .
MAMMALIAN GENOME, 2003, 14 (04) :231-241