Antilisterial activity of a Carnobacterium piscicola isolated from Brazilian smoked fish (Surubim [Pseudoplatystoma sp.]) and its activity against a persistent strain of Listeria monocytogenes isolated from Surubim

被引:25
作者
Alves, VF
De Martinis, ECP
Destro, MT
Vogel, BF
Gram, L
机构
[1] Danish Inst Fisheries Res, Dept Seafood Res, DK-2800 Lyngby, Denmark
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-14040903 Ribeirao Preto, Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508900 Sao Paulo, Brazil
关键词
D O I
10.4315/0362-028X-68.10.2068
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Data on the prevalence and growth of Listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. Our research describes L monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native Brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic DNA subtype as the strains that were isolated from the same factory 4 years earlier. A bacteriocinogenic strain of Carnobacterium piscicola (strain C2), isolated from vacuum-packed cold-smoked surubim, and two C. piscicola strains, isolated from vacuum-packed, cold-smoked salmon, were capable of limiting or completely inhibiting the growth of an L. monocytogenes (strain V2) isolated from surubim in fish peptone model systems incubated at 10 degrees C. Monocultures of L. monocytogenes reached 10(8) CFU/ml (g), whereas the growth of L. monocytogenes was completely inhibited by C piscicola C2. The bacteriocinogenic C piscicola A9b(+) and its nonbacteriocinogenic mutant A9b(-) reduced maximum Listeria levels by 2 to 3 log units. Both bacteriocinogenic C piscicola strains prevented listerial growth in cold-smoked fish juices (surubim and salmon). Although the carnobacteria grew poorly on cold-smoked surubim at 10 degrees C, the strains were able to reduce maximum Listeria counts by I to 3 log units in an artificially inoculated product (surubim). We conclude that Brazilian smoked fish products harbor L monocytogenes and should be stabilized against the growth of the organism. C. piscicola C2 has the potential for use as a bioprotective culture in surubim and other lightly preserved fish, but further studies are required to optimize its effect.
引用
收藏
页码:2068 / 2077
页数:10
相关论文
共 63 条
[1]  
*ANA, 2002, SUP PLAN REC HIDR
[2]   Prevalence of Listeria spp. in feces and carcasses at a lamb packing plant in Brazil [J].
Antoniollo, PC ;
Bandeira, FD ;
Jantzen, MM ;
Duval, EH ;
Da Silva, WP .
JOURNAL OF FOOD PROTECTION, 2003, 66 (02) :328-330
[3]  
Autio T, 1999, APPL ENVIRON MICROB, V65, P150
[4]   Protective cultures inhibit growth of Listeria monocytogenes and Escherichia coli O157:H7 in cooked, sliced, vacuum- and gas-packaged meat [J].
Bredholt, S ;
Nesbakken, T ;
Holck, A .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 53 (01) :43-52
[5]   A small outbreak of listeriosis associated with smoked mussels [J].
Brett, MSY ;
Short, P ;
McLauchlin, J .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1998, 43 (03) :223-229
[6]   Biodiversity of Listeria monocytogenes sensitivity to bacteriocin-producing Carnobacterium strains and application in sterile cold-smoked salmon [J].
Brillet, A ;
Pilet, MF ;
Prevost, H ;
Bouttefroy, A ;
Leroi, F .
JOURNAL OF APPLIED MICROBIOLOGY, 2004, 97 (05) :1029-1037
[7]  
BUCHANAN RL, 1992, J FOOD SAFETY, V12, P219, DOI 10.1111/j.1745-4565.1991.tb00080.x
[8]   Microbial competition: Effect of culture conditions on the suppression of Listeria monocytogenes Scott A by Carnobacterium piscicola [J].
Buchanan, RL ;
Bagi, LK .
JOURNAL OF FOOD PROTECTION, 1997, 60 (03) :254-261
[9]   Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats:: culture isolation, bacteriocin identification, and meat application experiments [J].
Budde, BB ;
Hornbæk, T ;
Jacobsen, T ;
Barkholt, V ;
Koch, AG .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 83 (02) :171-184
[10]   Occurrence of Listeria monocytogenes in food in Chile [J].
Cordano, AM ;
Rocourt, J .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 70 (1-2) :175-178