Biodistribution of arctigenin-loaded nanoparticles designed for multimodal imaging

被引：10

作者：

Cui, Qingxin ^{[1
]}

Hou, Yuanyuan ^{[1
]}

Wang, Yanan ^{[2
]}

Li, Xu ^{[3
]}

Liu, Yang ^{[1
]}

Ma, Xiaoyao ^{[1
]}

Wang, Zengyong ^{[1
]}

Wang, Weiya ^{[1
]}

Tao, Jin ^{[1
]}

Wang, Qian ^{[1
]}

Jiang, Min ^{[1
]}

Chen, Dongyan ^{[3
]}

Feng, Xizeng ^{[2
]}

Bai, Gang ^{[1
]}

机构：

[1] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

[2] Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

[3] Nankai Univ, Tianjin Key Lab Tumor Microenvironm & Neurovasc R, Sch Med, Dept Physiol, Tianjin 300071, Peoples R China

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2017年 / 15卷

基金：

中国国家自然科学基金;

关键词：

Nanoparticles; Self-assembly; Arctigenin; Localization; Target identification; ARCTIUM-LAPPA L; NATURAL-PRODUCTS; CHEMICAL BIOLOGY; SMALL MOLECULES; DRUG-DELIVERY; TARGET; IDENTIFICATION; LIGNAN; TUMOR; MICROENVIRONMENT;

D O I：

10.1186/s12951-017-0263-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Background: Tracking targets of natural products is one of the most challenging issues in fields ranging from pharmacognosy to biomedicine. It is widely recognized that the biocompatible nanoparticle (NP) could function as a "key" that opens the target "lock". Results: We report a functionalized poly-lysine NP technique that can monitor the target protein of arctigenin (ATG) in vivo non-invasively. The NPs were synthesized, and their morphologies and surface chemical properties were characterized by transmission electron microscopy (TEM), laser particle size analysis and atomic force microscopy (AFM). In addition, we studied the localization of ATG at the level of the cell and the whole animal (zebrafish and mice). We demonstrated that fluorescent NPs could be ideal carriers in the development of a feasible method for target identification. The distributions of the target proteins were found to be consistent with the pharmacological action of ATG at the cellular and whole-organism levels. Conclusions: The results indicated that functionalized poly-lysine NPs could be valuable in the multimodal imaging of arctigenin.

引用

页数：10

共 43 条

[1]

Biodistribution of biodegradable polymeric nano-carriers loaded with busulphan and designed for multimodal imaging [J].