CD34 cells from patients with trisomy 8 myelodysplastic syndrome (MDS) express early apoptotic markers but avoid programmed cell death by up-regulation of antiapoptotic proteins

被引：78

作者：

Sloand, Elaine M. ^{[1
]}

Pfannes, Loretta ^{[1
]}

Chen, Gubin ^{[1
]}

Shah, Simant ^{[1
]}

Solomou, Elena E. ^{[1
]}

Barrett, John ^{[1
]}

Young, Neal S. ^{[1
]}

机构：

[1] NHLBI, NIH, Bethesda, MD 20892 USA

来源：

BLOOD | 2007年 / 109卷 / 06期

关键词：

D O I：

10.1182/blood-2006-01-030643

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

CD34 cells from patients with trisomy 8 myelodysplastic syndrome (MDS) are distinguished from other MDS cells and from normal hematopoietic cells by their pronounced expression of apoptotic markers. Paradoxically, trisomy 8 clones can persist in patients with bone marrow failure and expand following immunosuppression. We previously demonstrated up-regulation of c-myc and CD1 by microarray analysis. Here, we confirmed these findings by real-time polymerase chain reaction (PCR), demonstrated up-regulation of survivin, c-myc, and CD1 protein expression, and documented comparable colony formation by annexin(+) trisomy 8(-) CD34(+) and annexin(-) CD34 cells. There were low levels of DNA degradation in annexin(+) trisomy 8 CD34 cells, which were comparable with annexin- CD34 cells. Trisomy 8 cells were resistant to apoptosis induced by gamma irradiation. Knock-down of survivin by siRNA resulted in preferential loss of trisomy 8 cells. These results suggest that trisomy 8 cells undergo incomplete apoptosis and are nonetheless capable of colony formation and growth.

引用

页码：2399 / 2405

页数：7

共 33 条

[1]

Bcl-x(L) can inhibit apoptosis in cells that have undergone Fas-induced protease activation [J].