Application of very short monolithic columns for separation of low and high molecular mass substances

被引：33

作者：

Podgornik, A

Barut, M

Jaksa, S

Jancar, J

Strancar, A

机构：

[1] BIA Separat DOO, SI-1000 Ljubljana, Slovenia

[2] Natl Inst Chem, SI-1000 Ljubljana, Slovenia

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2002年 / 25卷 / 20期

关键词：

monoliths; short columns; chromatographic columns; stationary phases; HETP; Z factors;

D O I：

10.1081/JLC-120016211

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Convective Interaction Media(R) (CIM) disk monolithic columns are specific among the chromatographic columns because of their monolithic structure and extremely short column length. In this work, HETP values and Z factors for different groups of molecules-proteins, DNA, oligonucleotides, peptides, and organic acids on strong anion exchange CIM disk monolithic columns were determined. Results are discussed in terms of the molecule structures and applied to develop different approaches for successful separation of abovementioned group of molecules on these types of columns.

引用

页码：3099 / 3116

页数：18

共 45 条

[1] CARRIER MEMBRANE AS A STATIONARY PHASE FOR AFFINITY-CHROMATOGRAPHY AND KINETIC-STUDIES OF MEMBRANE-BOUND ENZYMES [J].