Formulation of chitosan-TPP-pDNA nanocapsules for gene therapy applications

被引：56

作者：

Gaspar, V. M. ^{[1
]}

Sousa, F. ^{[1
]}

Queiroz, J. A. ^{[1
]}

Correia, I. J. ^{[1
]}

机构：

[1] Univ Beira Interior, Ctr Invest Ciencias Saude, Fac Ciencias Saude, P-6200506 Covilha, Portugal

来源：

NANOTECHNOLOGY | 2011年 / 22卷 / 01期

关键词：

DELIVERY-SYSTEM; IN-VITRO; TRANSFECTION EFFICIENCY; POLYMERIC NANOPARTICLES; DNA NANOPARTICLES; CELLULAR UPTAKE; DRUG-DELIVERY; DEACETYLATION; CARRIERS; CANCER;

D O I：

10.1088/0957-4484/22/1/015101

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

The encapsulation of DNA inside nanoparticles meant for gene delivery applications is a challenging process where several parameters need to be modulated in order to design nanocapsules with specific tailored characteristics. The purpose of this study was to investigate and improve the formulation parameters of plasmid DNA (pDNA) loaded in chitosan nanocapsules using tripolyphosphate (TPP) as polyanionic crosslinker. Nanocapsule morphology and encapsulation efficiency were analyzed as a function of chitosan degree of deacetylation and chitosan-TPP ratio. The manipulation of these parameters influenced not only the particle size but also the encapsulation and release of pDNA. Consequently the transfection efficiency of the nanoparticulated systems was also enhanced with the optimization of the particle characteristics. Overall, the differently formulated nanoparticulated systems possess singular properties that can be employed according to the desired gene delivery application.

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页数：12

相关论文

共 42 条

[1]

DNA-chitosan complexation: A dynamic light scattering study [J].

Alatorre-Meda, Manuel ;

Taboada, Pablo ;

Sabin, Juan ;

Krajewska, Barbara ;

Varela, Luis M. ;

Rodriguez, Julio R. .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2009, 339 (1-3) :145-152

[2]

BING H, 2007, J HUNAN AGR U NAT SC, V33, P353

[3]

Chitosan and chitosan ethylene oxide propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines [J].

Calvo, P ;

RemunanLopez, C ;

VilaJato, JL ;

Alonso, MJ .

PHARMACEUTICAL RESEARCH, 1997, 14 (10) :1431-1436

[4]

Atovaquone-loaded nanocapsules: influence of the nature of the polymer on their in vitro characteristics [J].

Cauchetier, E ;

Deniau, M ;

Fessi, H ;

Astier, A ;

Paul, M .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2003, 250 (01) :273-281

[5]

Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes [J].

Chithrani, B. Devika ;

Chan, Warren C. W. .

NANO LETTERS, 2007, 7 (06) :1542-1550

[6]

Sustained delivery and expression of DNA encapsulated in polymeric nanoparticles [J].

Cohen, H ;

Levy, RJ ;

Gao, J ;

Fishbein, I ;

Kousaev, V ;

Sosnowski, S ;

Slomkowski, S ;

Golomb, G .

GENE THERAPY, 2000, 7 (22) :1896-1905

[7]

Ionically crosslinked chitosan/tripolyphosphate nanoparticles for oligonucleotide and plasmid DNA delivery [J].

Csaba, Noemi ;

Koeping-Hoeggard, Magnus ;

Jose Alonso, Maria .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2009, 382 (1-2) :205-214

[8]

Improved barrier and mechanical properties of novel hydroxypropyl methylcellulose edible films with chitosan/tripolyphosphate nanoparticles [J].

de Moura, Marcia R. ;

Aouada, Fauze A. ;

Avena-Bustillos, Roberto J. ;

McHugh, Tara H. ;

Krochta, John M. ;

Mattoso, Luiz H. C. .

JOURNAL OF FOOD ENGINEERING, 2009, 92 (04) :448-453

[9]

Cationic polymer based gene delivery systems [J].

De Smedt, SC ;

Demeester, J ;

Hennink, WE .

PHARMACEUTICAL RESEARCH, 2000, 17 (02) :113-126

[10]

In vitro and in vivo evaluations of PLGA microsphere vaccine formulations containing pDNA coexpressing Hepatitis B surface antigen and Interleukin-2 [J].

Eratalay, Aydan ;

Coskun-Ari, F. Filiz ;

Oener, Filiz ;

Oezcengiz, Erkan .

JOURNAL OF MICROENCAPSULATION, 2010, 27 (01) :48-56

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