Counting the number of proteins coupled to single nanoparticles

被引：76

作者：

Casanova, Didier

Giaume, Dornitille

Moreau, Melanie

Martin, Jean-Louis

Gacoin, Thierry

Boilot, Jean-Pierre

Alexandrou, Antigoni ^{[1
]}

机构：

[1] Ecole Polytech, Lab Opt & Biosci, CNRS, F-91128 Palaiseau, France

[2] Ecole Polytech, Phys Mat Condensee Lab, CNRS, F-91128 Palaiseau, France

[3] INSERM, U696, F-91128 Palaiseau, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 42期

关键词：

D O I：

10.1021/ja0731975

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We implemented amine coating of lanthanide-ion doped oxide luminescent nanoparticles and coupling to a protein labeled with an organic fluorophore. We exploited the stepwise photobleaching of the organic fluorophores and their initial emission to count the number of proteins coupled to single nanoparticles. We thus precisely measured the distribution of the protein-nanoparticle ratio and showed that its maximum is different from the average ratio determined from ensemble measurements. The accurate quantification of the biomolecule-particle coupling opens up the possibility of selecting finely controlled conjugates.

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页码：12592 / +

页数：3

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