Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

被引：6

作者：

Morita, Hiroyuki ^{[2
]}

Tanio, Michikazu ^{[2
]}

Kondo, Shin ^{[1
]}

Kato, Ryohei ^{[1
]}

Wanibuchi, Kiyofumi ^{[3
]}

Noguchi, Hiroshi ^{[3
]}

Sugio, Shigetoshi ^{[1
]}

Abe, Ikuro ^{[3
,4
]}

Kohno, Toshiyuki ^{[2
]}

机构：

[1] Mitsubishi Chem Corp, Innovat Ctr Yokohama, Aoba Ku, Kanagawa 2278502, Japan

[2] Mitsubishi Kagaku Inst Life Sci MITILS, Tokyo 1948511, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan

[4] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷

关键词：

D O I：

10.1107/S1744309108006775

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 angstrom, alpha = gamma = 90.0, beta = 100.5 degrees. Diffraction data were collected to 1.8 angstrom resolution using synchrotron radiation at BL24XU of SPring-8.

引用

页码：304 / 306

页数：3

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