Musclin inhibits insulin activation of Akt/protein kinase B in rat skeletal muscle

被引：54

作者：

Liu, Y. ^{[1
]}

Huo, X. ^{[2
]}

Pang, X. F. ^{[3
]}

Zong, Z. H. ^{[4
]}

Meng, X. ^{[1
]}

Liu, G. L. ^{[1
]}

机构：

[1] China Med Univ, Dept Endocrinol, Hosp 1, Shenyang 110001, Liaoning, Peoples R China

[2] China Med Univ, Dept Gen Surg 3, Hosp 1, Shenyang 110001, Liaoning, Peoples R China

[3] China Med Univ, Dept Cardiol, Hosp 1, Shenyang 110001, Liaoning, Peoples R China

[4] China Med Univ, Dept Biochem & Mol Biol, Shenyang 110001, Liaoning, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2008年 / 36卷 / 03期

关键词：

rosiglitazone; thiazolidinedione; diabetes mellitus; rats; musclin; insulin resistance; PPAR gamma; LXR alpha; PI3K; AkT/PKB;

D O I：

10.1177/147323000803600314

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Musclin is a muscle-derived secretory peptide that induces insulin resistance in vitro. We studied the effect of musclin (0.5 mu g/ml) on insulin-stimulated glucose uptake in rat skeletal muscles and also the effect of rosiglitazone (0.4 mu g/ml). Preincubation of muscles with musclin resulted in decreased insulin-stimulated glucose uptake. Musclin also reduced expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) and liver X receptor a (LXR alpha) mRNAs, although expression of glucose transporter 4 mRNA was unaltered. Rosiglitazone attenuated the effects of musclin on glucose uptake and PPAR gamma and LXR alpha mRNA expression. Western blotting demonstrated that activation of protein kinase B (Akt/PKB) in the insulin-signalling cascade was decreased by musclin but corrected by rosiglitazone. These findings suggest that musclin-induced impairment of insulin-stimulated glucose uptake in skeletal muscle is related to Akt/PKB inhibition and might be modulated by PPAR gamma/ LXR alpha.

引用

页码：496 / 504

页数：9

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