Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae

被引:174
作者
Regenberg, B
Düring-Olsen, L
Kielland-Brandt, MC [1 ]
Holmberg, S
机构
[1] Carlsberg Lab, Dept Yeast Genet, DK-2500 Copenhagen, Denmark
[2] Univ Copenhagen, Inst Mol Biol, Dept Genet, DK-1353 Copenhagen K, Denmark
关键词
amino-acid uptake; transporter; yeast;
D O I
10.1007/s002940050506
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
All known amino-acid permeases (AAPs) in Saccharomyces cerevisiae belong to a single family of homologous proteins. Genes of 15 AAPs were overexpressed in different strains, and the ability to take up one or more of the 20 common L-alpha-amino acids was studied in order to obtain a complete picture of the substrate specificity for these permeases. Radiolabelled aminoacid uptake measurements showed that Agp1p is a general permease for most uncharged amino acids (Ala, Gly, Ser, Thr, Cys, Met, Phe, Tyr, lie, Leu, Val, Gin and Asn). Gnp1p, which is closely related to Agp1p, has a somewhat less-broad specificity, transporting Leu, Ser, Thr, Cys, Met, Gin and Asn, while Bap2p and Bap3p, which are also closely related to Agp1p, are able to transport Ile, Leu, Val, Cys, Met, Phe, Tyr and Trp. All four permeases are transcriptionally induced by an extracellular amino acid, but differ in expression with respect to the nitrogen source. On a non-repressive nitrogen source, AGP1 is induced, while GLN1, BAP2 and BAP3 are not. Except for Dip5p, which is a transporter for Glu, Asp, Gin, Asn, Ser, Ala and Gly, the rest of the permeases exhibit narrow specificity. Tat2p can take up Phe, Trp and Tyr, Put4p can transport Ala, Gly and Pro; while Can1p, Lyp1p and the previously uncharacterized Alp1p are specific for the cationic amino acids. These findings modify the prevalent view that S. cerevisiae only contains one general amino-acid permease, Gap1p, and a number of permeases that are specific for a single or a few amino acids.
引用
收藏
页码:317 / 328
页数:12
相关论文
共 59 条
[1]   YEAST ARGININE PERMEASE - NUCLEOTIDE-SEQUENCE OF THE CAN1 GENE [J].
AHMAD, M ;
BUSSEY, H .
CURRENT GENETICS, 1986, 10 (08) :587-592
[2]   An overview of membrane transport proteins in Saccharomyces cerevisiae [J].
Andre, B .
YEAST, 1995, 11 (16) :1575-1611
[3]  
ANDRE B, 1993, MOL GEN GENET, V237, P17
[4]   TATI encodes a low-affinity histidine transporter in Saccharomyces cerevisiae [J].
Bajmoczi, M ;
Sneve, M ;
Eide, DJ ;
Drewes, LR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 243 (01) :205-209
[5]  
BALLARINDENTI A, 1984, BIOCHIM BIOPHYS ACTA, V778, P1, DOI 10.1016/0005-2736(84)90442-5
[6]   AMMONIA REGULATION OF AMINO-ACID PERMEASES IN SACCHAROMYCES-CEREVISIAE [J].
COURCHESNE, WE ;
MAGASANIK, B .
MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (04) :672-683
[7]   REGULATION OF HISTIDINE UPTAKE BY SPECIFIC FEEDBACK INHIBITION OF 2 HISTIDINE PERMEASES IN SACCHAROMYCES-CEREVISIAE [J].
CRABEEL, M ;
GRENSON, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 14 (01) :197-&
[8]   EVIDENCE FOR 3 GLUTAMIC-ACID TRANSPORTING SYSTEMS WITH SPECIALIZED PHYSIOLOGICAL FUNCTIONS IN SACCHAROMYCES-CEREVISIAE [J].
DARTE, C ;
GRENSON, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1975, 67 (03) :1028-1033
[9]   REGULATORY CIRCUIT FOR RESPONSES OF NITROGEN CATABOLIC GENE-EXPRESSION TO THE GLN3-PROTEIN AND DAL80-PROTEIN AND NITROGEN CATABOLITE REPRESSION IN SACCHAROMYCES-CEREVISIAE [J].
DAUGHERTY, JR ;
RAI, R ;
ELBERRY, HM ;
COOPER, TG .
JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :64-73
[10]   Regulation of expression of the amino acid transporter gene BAP3 in Saccharomyces cerevisiae [J].
de Boer, M ;
Bebelman, JP ;
Gonçalves, PM ;
Maat, J ;
van Heerikhuizen, H ;
Planta, RJ .
MOLECULAR MICROBIOLOGY, 1998, 30 (03) :603-613