Population research of genetic polymorphism at amino acid position 631 in chicken mx protein with differential antiviral activity

被引：38

作者：

Seyama, T.

Ko, J. H.

Ohe, M.

Sasaoka, N.

Okada, A.

Gomi, H.

Yoneda, A.

Ueda, J.

Nishibori, M.

Okamoto, S.

Maeda, Y.

Watanabe, T. ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan

[2] Hiroshima Univ, Lab Anim Breeding & Genet, Higashihiroshima 7398528, Japan

来源：

BIOCHEMICAL GENETICS | 2006年 / 44卷 / 9-10期

关键词：

chicken; jungle fowl; Mx gene; polymorphism; antiviral gene; PCR-RFLP;

D O I：

10.1007/s10528-006-9040-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A single amino acid substitution between Asn and Ser at position 631 in the chicken Mx protein has been reported to determine resistant and sensitive antiviral activity. In this study, we investigate whether various kinds of chicken breeds and jungle fowls carry the resistant or sensitive Mx allelic gene by using the mismatched PCR-restriction fragment length polymorphism (RFLP) technique. In total, 271 samples from 36 strains of 17 chicken breeds and from 3 kinds of jungle fowls were examined. The rates of the resistant Mx gene and sensitive gene were 59.2% and 40.8%, respectively. Only a Red jungle fowl captured in Laos carried the resistant Mx gene, and the other three Red jungle fowls from Indonesia and Gray and Green jungle fowls all had the sensitive Mx gene. These results were confirmed by the determination of amino acid sequences in the GTPase effector domain of jungle fowls.

引用

页码：437 / 448

页数：12

共 26 条

[1] CDNA STRUCTURES AND REGULATION OF 2 INTERFERON-INDUCED HUMAN MX PROTEINS [J].