Exposure to the herbicide acetochlor alters thyroid hormone-dependent gene expression and metamorphosis in Xenopus laevis

被引:145
作者
Crump, D
Werry, K
Veldhoen, N
Van Aggelen, G
Helbing, CC
机构
[1] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada
[2] Pacific Environm Sci Ctr, N Vancouver, BC, Canada
关键词
acetochlor; cDNA array; endocrine disruptor; gene expression; herbicides; metamorphosis; persistent organic pollutants; tadpoles; thyroid hormone; Xenopus laevis;
D O I
10.1289/ehp.021101199
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A growing number of substances released into the environment disrupt normal endocrine mechanisms in a wide range of vertebrates. Little is known about the effects and identities of endocrine-disrupting chemicals (EDCs) that target thyroid hormone (TH) action, particularly at the cellular level. Frog tadpole metamorphosis depends completely on TH, which has led to the suggestion of a metamorphosis-based assay for screening potential EDCs. A major mechanism of TH action is the alteration of gene expression via hormone-bound nuclear receptors. To assess the gene expression profiles in the frog model, we designed a novel multispecies frog cDNA microarray. Recently, the preemergent herbicide acetochlor was shown to accelerate 3,5,3'-triiodothyronine (T-3)-induced forelimb emergence and increase mRNA expression of thyroid hormone beta receptors in ranid tadpoles. Here we show that T-3-induced metamorphosis of Xenopus laevis, a species commonly used in the laboratory, is accelerated upon acute exposure to an environmentally relevant level of acetochlor. The morphologic changes observed are preceded by alterations in gene expression profiles detected in the tadpole tail, and the nature of these profiles suggest a novel mechanism of action for acetochlor.
引用
收藏
页码:1199 / 1205
页数:7
相关论文
共 60 条
[1]   IDENTIFICATION OF NUCLEAR PREREPLICATION CENTERS POISED FOR DNA-SYNTHESIS IN XENOPUS EGG EXTRACTS - IMMUNOLOCALIZATION STUDY OF REPLICATION PROTEIN-A [J].
ADACHI, Y ;
LAEMMLI, UK .
JOURNAL OF CELL BIOLOGY, 1992, 119 (01) :1-15
[2]  
Amano T, 1998, Wound Repair Regen, V6, P302, DOI 10.1046/j.1524-475X.1998.60406.x
[3]   Evaluation of the potential carcinogenicity and genetic toxicity to humans of the herbicide acetochlor [J].
Ashby, J ;
Kier, L ;
Wilson, AGE ;
Green, T ;
Lefevre, PA ;
Tinwell, H ;
Willis, GA ;
Heydens, WF ;
Clapp, MJL .
HUMAN & EXPERIMENTAL TOXICOLOGY, 1996, 15 (09) :702-735
[4]  
ASTM (American Society for Testing and Materials), 2000, STAND GUID COND AC T
[5]  
Barbash JE, 1999, DISTRIBUTION MAJOR H
[6]   Applications of gene arrays in environmental toxicology:: Fingerprints of gene regulation associated with cadmium chloride, benzo(a)pyrene, and trichloroethylene [J].
Bartosiewicz, M ;
Penn, S ;
Buckpitt, A .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 (01) :71-74
[7]   THE TYPE-III 5-DEIODINASE IN RANA-CATESBEIANA TADPOLES IS ENCODED BY A THYROID HORMONE-RESPONSIVE GENE [J].
BECKER, KB ;
SCHNEIDER, MJ ;
DAVEY, JC ;
GALTON, VA .
ENDOCRINOLOGY, 1995, 136 (10) :4424-4431
[8]   The type 2 and type 3 iodothyronine deiodinases play important roles in coordinating development in Rana catesbeiana tadpoles [J].
Becker, KB ;
Stephens, KC ;
Davey, JC ;
Schneider, MJ ;
Galton, VA .
ENDOCRINOLOGY, 1997, 138 (07) :2989-2997
[9]   The thyroid hormone-induced tail resorption program during Xenopus laevis metamorphosis [J].
Brown, DD ;
Wang, Z ;
Furlow, JD ;
Kanamori, A ;
Schwartzman, RA ;
Remo, BF ;
Pinder, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (05) :1924-1929
[10]  
BUCKBINDER L, 1992, J BIOL CHEM, V267, P25786