PYK2/CAKβ represents a redox-sensitive tyrosine kinase in vascular smooth muscle cells

被引：50

作者：

Frank, GD

Motley, ED

Inagami, T

Eguchi, S ^{[1
]}

机构：

[1] Vanderbilt Univ, Med Ctr, Sch Med, Dept Biochem, Nashville, TN 37232 USA

[2] Meharry Med Coll, Dept Anat & Physiol, Nashville, TN 37208 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 03期

关键词：

tyrosine kinase; PYK2; H2O2; reactive oxygen species; vascular smooth muscle cell; angiotensin II;

D O I：

10.1006/bbrc.2000.2505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vascular smooth muscle cells (VSMCs), the focal adhesion kinase-related tyrosine kinase PYK2/CAK beta is activated by vascular mitogens, Because reactive oxygen species (ROS) are assumed to mediate mitogenic signals by these agonists, we examined the possible link between ROS and PYK2 in cultured rat VSMCs. Here we present several lines of evidence showing that PYK2 is activated by ROS in VSMCs. The inhibitory effect of an antioxidant, N-acetyl-cysteine, on PYK2 activation by its specific agonists further suggests the pivotal role of PYK2 in vascular remodeling associated with enhanced ROS production. (C) 2000 Academic Press.

引用

收藏

页码：761 / 765

页数：5

相关论文

共 30 条

[1] Reactive oxygen species as mediators of signal transduction in cardiovascular disease [J].

Abe, J ;

Berk, BC .

TRENDS IN CARDIOVASCULAR MEDICINE, 1998, 8 (02) :59-64

[2] Fyn and JAK2 mediate Ras activation by reactive oxygen species [J].

Abe, J ;

Berk, BC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (30) :21003-21010

[3] Oxidative stress activates extracellular signal-regulated kinases through Src and ras in cultured cardiac myocytes of neonatal rats [J].

Aikawa, R ;

Komuro, I ;

Yamazaki, T ;

Zou, YZ ;

Kudoh, S ;

Tanaka, M ;

Shiojima, I ;

Hiroi, Y ;

Yazaki, Y .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (07) :1813-1821

[4] IDENTIFICATION AND CHARACTERIZATION OF A NOVEL RELATED ADHESION FOCAL TYROSINE KINASE (RAFTK) FROM MEGAKARYOCYTES AND BRAIN [J].

AVRAHAM, S ;

LONDON, R ;

FU, YG ;

OTA, S ;

HIREGOWDARA, D ;

LI, JZ ;

JIANG, SX ;

PASZTOR, LN ;

WHITE, RA ;

GROOPMAN, JE ;

AVRAHAM, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (46) :27742-27751

[5] Regulation of a calcium-dependent tyrosine kinase in vascular smooth muscle cells by angiotensin II and platelet-derived growth factor - Dependence on calcium and the actin cytoskeleton [J].

Brinson, AE ;

Harding, T ;

Diliberto, PA ;

He, YQ ;

Li, X ;

Hunter, D ;

Herman, B ;

Earp, HS ;

Graves, LM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (03) :1711-1718

[6] A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activation [J].

Dikic, I ;

Tokiwa, G ;

Lev, S ;

Courtneidge, SA ;

Schlessinger, J .

NATURE, 1996, 383 (6600) :547-550

[7] Involvement of PYK2 in angiotensin II signaling of vascular smooth muscle cells [J].

Eguchi, S ;

Iwasaki, H ;

Inagami, T ;

Numaguchi, K ;

Yamakawa, T ;

Motley, ED ;

Owada, KM ;

Marumo, F ;

Hirata, Y .

HYPERTENSION, 1999, 33 (01) :201-206

[8] Calcium-dependent epidermal growth factor receptor transactivation mediates the angiotensin II-induced mitogen-activated protein kinase activation in vascular smooth muscle cells [J].

Eguchi, S ;

Numaguchi, K ;

Iwasaki, H ;

Matsumoto, T ;

Yamakawa, T ;

Utsunomiya, H ;

Motley, ED ;

Kawakatsu, H ;

Owada, KM ;

Hirata, Y ;

Marumo, F ;

Inagami, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) :8890-8896

[9] Identification of an essential signaling cascade for mitogen-activated protein kinase activation by angiotensin II in cultured rat vascular smooth muscle cells - Possible requirement of G(q)-mediated p21(ras) activation coupled to a Ca2+/calmodulin-sensitive tyrosine kinase [J].

Eguchi, S ;

Matsumoto, T ;

Motley, ED ;

Utsunomiya, H ;

Inagami, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (24) :14169-14175

[10]

FIALKOW L, 1994, J BIOL CHEM, V269, P31234