A spectrophotometric assay for nitrate in an excess of nitrite

被引：65

作者：

Beda, N ^{[1
]}

Nedospasov, A ^{[1
]}

机构：

[1] Inst Mol Genet, Moscow, Russia

来源：

NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2005年 / 13卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

nitrite; nitrate; nitric oxide; Griess reaction; vanadium (III) chloride; spectrophotometric determination;

D O I：

10.1016/j.niox.2005.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Miranda et al. have developed a method for simultaneous evaluation of nitrate and nitrite concentrations using reduction of nitrate by vanadium(III) combined with detection by the acidic Griess reaction [K.M. Miranda, M.G. Espey, D.A. Wink, A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite, Nitric Oxide 5 (2001) 62-71]. The sensitivity of the nitrate assay decline if the mixture analyzed contains a large excess of nitrite relative to nitrate, for instance, in the case of oxidation products of nitric oxide (NO) in aerated solutions, or in sweat. By this reason nitrite should be removed before the nitrate assay, if [NO2-] >> [NO3-]. Here we lay out an improved method allowing the above limitation to be erased, using sulfamic acid for nitrite removal. We also describe some modifications that enhance the reproducibility of the assay. (c) 2005 Published by Elsevier Inc.

引用

页码：93 / 97

页数：5

共 4 条

[1]

OXIDATION OF NITRIC-OXIDE IN AQUEOUS-SOLUTION TO NITRITE BUT NOT NITRATE - COMPARISON WITH ENZYMATICALLY FORMED NITRIC-OXIDE FROM L-ARGININE [J].