Poliovirus 5′-terminal cloverleaf RNA is required in cis for VPg uridylylation and the initiation of negative-strand RNA synthesis

被引:82
作者
Lyons, T
Murray, KE
Roberts, AW
Barton, DJ
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Microbiol, Denver, CO 80262 USA
[2] Univ Colorado, Hlth Sci Ctr, Program Mol Biol, Denver, CO 80262 USA
关键词
D O I
10.1128/JVI.75.22.10696-10708.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chimeric poliovirus RNAs, possessing the 5' nontranslated region (NTR) of hepatitis C virus in place of the 5' NTR of poliovirus, were used to examine the role of the poliovirus 5' NTR in viral replication. The chimeric viral RNAs were incubated in cell-free reaction mixtures capable of supporting the sequential translation and replication of poliovirus RNA. Using preinitiation RNA replication complexes formed in these reactions, we demonstrated that the 3' NTR of poliovirus RNA was insufficient, by itself, to recruit the viral replication proteins required for negative-strand RNA synthesis. The 5'-terminal cloverleaf of poliovirus RNA was required in cis to form functional preinitiation RNA replication complexes capable of uridylylating VPg and initiating the synthesis of negative-strand RNA. These results are consistent with a model in which the 5'-terminal cloverleaf and 3' NTRs of poliovirus RNA interact via temporally dynamic ribonucleoprotein complexes to coordinately mediate and regulate the sequential translation and replication of poliovirus RNA.
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收藏
页码:10696 / 10708
页数:13
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