Adsorption of bovine serum albumin on nanosized magnetic particles

被引：302

作者：

Peng, ZG ^{[1
]}

Hidajat, K ^{[1
]}

Uddin, MS ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Environm Engn, Singapore 119260, Singapore

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2004年 / 271卷 / 02期

关键词：

nanosized magnetic particles; bovine serum albumin; zeta potential; adsorption; desorption;

D O I：

10.1016/j.jcis.2003.12.022

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Adsorption of bovine serum albumin (BSA) on nanosized magnetic particles (Fe3O4) was carried out in the presence of carbodiimide. The equilibrium and kinetics of the adsorption process were studied. Nanosized magnetic particles (Fe3O4) were prepared by the chemical precipitation method using Fe2+, Fe3+ salts, and ammonium hydroxide under a nitrogen atmosphere. Characterizations of magnetic particles were carried out using transmission electron microscopy (TEM) and a vibrating sample magnetometer (VSM). Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to confirm the attachment of BSA on magnetic particles. Effects of pH and salt concentrations were investigated on the adsorption process. The experimental results show that the adsorption of BSA on magnetic particles was affected greatly by the pH, while the effect of salt concentrations was insignificant at a low concentration range. The adsorption equilibrium isotherm was fitted well by the Langmuir model. The maximum adsorption of BSA on magnetic particles occurred at the isoelectric point of BSA. Adsorption kinetics was analyzed by a linear driving force mass-transfer model. BSA was desorbed from magnetic particles under alkaline conditions, which was confirmed by SDS-PAGE electrophoresis and FTIR results. (C) 2004 Published by Elsevier Inc.

引用

页码：277 / 283

页数：7

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