Unraveling the mechanism of BRCA2 in homologous recombination

被引：165

作者：

Holloman, William K. ^{[1
]}

机构：

[1] Weill Cornell Med Coll, Weill Cornell Canc Ctr, Dept Microbiol & Immunol, New York, NY USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 07期

基金：

美国国家卫生研究院;

关键词：

CANCER SUSCEPTIBILITY GENE; STRAND-BREAK REPAIR; RAD51 FILAMENT FORMATION; D-LOOP FORMATION; DNA-REPAIR; USTILAGO-MAYDIS; RADIATION HYPERSENSITIVITY; IONIZING-RADIATION; VERTEBRATE CELLS; BRH2; PROTEIN;

D O I：

10.1038/nsmb.2096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BRCA2 is the product of a breast cancer susceptibility gene in humans and the founding member of an emerging family of proteins present throughout the eukaryotic domain that serve in homologous recombination. The function of BRCA2 in recombination is to control RAD51, a protein that catalyzes homologous pairing and DNA strand exchange. By physically interacting with both RAD51 and single-stranded DNA, BRCA2 mediates delivery of RAD51 preferentially to sites of single-stranded DNA (ssDNA) exposed as a result of DNA damage or replication problems. Through its action, BRCA2 helps restore and maintain integrity of the genome. This review highlights recent studies on BRCA2 and its orthologs that have begun to illuminate the molecular mechanisms by which these proteins control homologous recombination.

引用

页码：748 / 754

页数：7

共 87 条

[1] Double-strand break repair deficiency and radiation sensitivity in BRCA2 mutant cancer cells [J].

Abbott, DW ;

Freeman, ML ;

Holt, JT .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1998, 90 (13) :978-985

[2]

Ayoub Nabieh, 2009, Curr Biol, V19, P1075, DOI 10.1016/j.cub.2009.05.057

[3] Synergistic actions of Rad51 and Rad52 in recombination and DNA repair [J].

Benson, FE ;

Baumann, P ;

West, SC .

NATURE, 1998, 391 (6665) :401-404

[4] The BRC repeats are conserved in mammalian BRCA2 proteins [J].

Bignell, G ;

Micklem, G ;

Stratton, MR ;

Ashworth, A ;

Wooster, R .

HUMAN MOLECULAR GENETICS, 1997, 6 (01) :53-58

[5] From RPA to BRCA2: lessons from single-stranded DNA binding by the OB-fold [J].

Bochkarev, A ;

Bochkareva, E .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2004, 14 (01) :36-42

[6] Internal repeats in the BRCA2 protein sequence [J].

Bork, P ;

Blomberg, N ;

Nilges, M .

NATURE GENETICS, 1996, 13 (01) :22-23

[7] Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase [J].

Bryant, HE ;

Schultz, N ;

Thomas, HD ;

Parker, KM ;

Flower, D ;

Lopez, E ;

Kyle, S ;

Meuth, M ;

Curtin, NJ ;

Helleday, T .

NATURE, 2005, 434 (7035) :913-917

[8] Mechanisms of Dealing with DNA Damage-Induced Replication Problems [J].

Budzowska, Magda ;

Kanaar, Roland .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2009, 53 (01) :17-31

[9] Rad54 dissociates homologous recombination intermediates by branch migration [J].

Bugreev, Dmitry V. ;

Hanaoka, Fumio ;

Mazin, Alexander V. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (08) :746-753

[10] Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity [J].

Bugreev, DV ;

Mazin, AV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (27) :9988-9993

← 1 2 3 4 5 6 7 8 9 →