Resveratrol attenuates high-fat diet-induced insulin resistance by influencing skeletal muscle lipid transport and subsarcolemmal mitochondrial β-oxidation

被引:70
作者
Chen, Lu-Lu [1 ]
Zhang, Hao-Hao [1 ]
Zheng, Juan [1 ]
Hu, Xiang [1 ]
Kong, Wen [1 ]
Hu, Di [1 ]
Wang, Su-Xing [1 ]
Zhang, Ping [1 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Endocrinol, Wuhan 430022, Peoples R China
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 2011年 / 60卷 / 11期
基金
中国国家自然科学基金;
关键词
METABOLIC SYNDROME; ACID TRANSPORT; SIRT1; RATS; EXPRESSION; MICE; PGC-1-ALPHA; SENSITIVITY; DYSFUNCTION; ACTIVATION;
D O I
10.1016/j.metabol.2011.04.002
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Although resveratrol (RES) is implicated in the regulation of insulin sensitivity in rodents, the exact mechanism underlying this effect remains unclear. Therefore, we sought to investigate how RES affects skeletal muscle lipid transportation and lipid oxidation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondrial populations in high-fat diet (HFD)-induced insulin resistance (IR) rats. Systemic and skeletal muscle insulin sensitivity together with expressions of several genes related to mitochondrial biogenesis and skeletal muscle lipid transportation was studied in rats fed a normal diet, an HFD, and an HFD with intervention of RES for 8 weeks. Citrate synthase (CS), electron transport chain (ETC) activities, and several enzymes for mitochondrial beta-oxidation were assessed in SS and IMF mitochondria from tibialis anterior muscle. The HFD-fed rats exhibited obvious systemic and skeletal muscle IR as well as intramuscular lipid accumulation. SIRT1 activity and expression of genes related to mitochondrial biogenesis were greatly declined, whereas the gene for lipid transportation, FAT/CD36, was upregulated (P < .05). Subsarcolemmal but not IMF mitochondria displayed lower CS, ETC, and beta-oxidation activities. By contrast, RES treatment protected rats against diet-induced intramuscular lipid accumulation and IR, increased SIRT1 activity and mitochondrial biogenesis, and reverted the decline in SS mitochondrial CS and ETC activities. Importantly, although expression of FAT/CD36 was increased (11%, P < .05), activities of SS mitochondrial beta-oxidation enzymes were largely enhanced (41%similar to 67%, P < .05). This study suggests that RES ameliorates insulin sensitivity consistent with an improved balance between skeletal muscle lipid transportation and SS mitochondrial beta-oxidation in HFD rats. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:1598 / 1609
页数:12
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