Fasciola gigantica:: Purification and characterization of adenosine deaminase

被引：8

作者：

Ali, Ehab M. M. ^{[1
]}

机构：

[1] Tanta Univ, Fac Sci, Dept Chem, Div Biochem, Tanta, Egypt

来源：

EXPERIMENTAL PARASITOLOGY | 2008年 / 119卷 / 02期

关键词：

Fasciola gigantica; nucleotidase; adenosine; adenosine deaminase; purification;

D O I：

10.1016/j.exppara.2008.03.004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学]; 100103 [病原生物学];

摘要：

Nucleotidase cascades (apyrase, 5' nucleotidase, and adenosine deaminase (ADA) were investigated in the parasitic trematode Fasciola gigantica. ADA had the highest activity in the nucleotidase cascades. Adenosine deaminase was purified from F gigantica through acetone precipitation and chromatography on CM-cellulose. Two forms of enzyme (ADAI, ADAII) were separated. ADAII was purified to homogeneity after chromatography on Sephacryl S-200. The molecular mass was 29 KDa for the native and denatured enzyme using gel filtration and SDS-PAGE, respectively. The enzyme (ADAII) had a pH optimum at 7.5 and a K-m 1.0 mM adenosine, a temperature optimum at 40 degrees C and heat stability up to 40 degrees C. The order of effectiveness of metals as inhibitors was found to be Hg2+ > Mn2+ > Cu2+ > Ca2+ > Zn2+ >Ni2+ > Ba2+ (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：285 / 290

页数：6

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