Separate metal requirements for loop interactions and catalysis in the extended hammerhead ribozyme

被引：43

作者：

Kim, NK ^{[1
]}

Murali, A ^{[1
]}

DeRose, VJ ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 41期

关键词：

D O I：

10.1021/ja0541027

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An extended hammerhead ribozyme derived from Schistosoma mansoni, including conserved loops in stems I and II, has been examined to directly monitor the relationship between docking of loops and its activity using site-directed spin labeling (SDSL) and EPR spectroscopy. Dynamics with EPR spectroscopy and fast-quench kinetics measurements have shown that the docking of stems I and II occurs at low Mg2+ concentrations ([Mg2+]1/2,dock = 0.7 mM, 0.1 M NaCl), but a much weaker Mg2+ interaction ([Mg2+]1/2,cat ∼90 mM) increases activity to very high maximum rates of ∼1 s-1 at 0.1 M Na+ and pH 7.0. Copyright © 2005 American Chemical Society.

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页码：14134 / 14135

页数：2

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