Enantiomeric resolution of some imidazole antifungal agents on Chiralpak WH chiral stationary phase using HPLC

The enantiomeric resolution of (+/-)-econazole, (+/-)-miconazole and (+/-)-sulconazole was achieved on a Chiralpak WH column. The mobile phase used was hexane-2-propanol-diethylamine (400:99:1, v/v/v). The flow rates of the mobile phase used were 0.50 and 1.00 mL min(-1). The values of a of the resolved enantiomers of econazole, miconazole and sulconazole were in the range of 1.68 to 1.23 while the values of Rs varied from 2.42 to 1.10. The resolution of these antifungal agents on Chiralpak WH column is governed by ligand exchange mechanism. Hydrophobic interactions also play an important role for the enantiomeric resolution of antifungal agents on the reported CSP.