Genetic analysis of the ycgJ-metB-cysK-ygaG operon negatively regulated by the VirR/VirS system in Clostridium perfringens

被引：21

作者：

Ohtani, K ^{[1
]}

Takamura, H ^{[1
]}

Yaguchi, H ^{[1
]}

Hayashi, H ^{[1
]}

Shimizu, T ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Basic Med Sci, Dept Microbiol, Tsukuba, Ibaraki 3058575, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2000年 / 44卷 / 06期

关键词：

Clostridium perfringens; two-component system; genome analysis; metB-cysK;

D O I：

10.1111/j.1348-0421.2000.tb02528.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The 5'-flanking region of the metB-cysK-ygaG operon, whose expression is negatively regulated by the VirR/VirS system in C, perfringens, was analyzed. The region contained the cygJ, mscL, and colA genes encoding a hypothetical protein, a large conductance mechanosensitive channel protein, and kappa-toxin (collagenase), respectively, Northern analysis revealed that the ycgJ gene was transcribed as a 4.9-kb operon together with the metB-cysK-ygaG genes and that this operon was negatively regulated by the VirR/VirS system. It is indicated that the pfoA (theta-tor;in or perfringolysin O), colA, and ycgJ-metB-cysK-ygaG genes that belong to the VirR/VirS regulon are situated very close together in a 26.5-kb region of the chromosome, but do not form a pathogenic island.

引用

页码：525 / 528

页数：4

共 18 条

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