Overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus (Magnaporthe grisea) in transgenic rice

被引：114

作者：

Kanzaki, H

Nirasawa, S

Saitoh, H

Ito, M

Nishihara, M

Terauchi, R

Nakamura, I

机构：

[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan

[2] Chiba Univ, Grad Sch Sci & Technol, Matsudo, Chiba 2710092, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 105卷 / 6-7期

关键词：

rice; defensin; rice blast fungus; Agrobacterium tumefaciens;

D O I：

10.1007/s00122-001-0817-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Transgenic rice (Oryza sativa cv. Sasanishiki) overexpressing the wasabi defensin gene, a plant defensin effective against the rice blast fungus, was generated by Agrobacterium tumefaciens-mediated transformation. Twenty-two T2 homozygous lines harboring the wasabi defensin gene were challenged by the blast fungus. Transformants exhibited resistance to rice blast at various levels. The inheritance of the resistance over generations was investigated. T3 plants derived from two highly blast-resistant T2 lines (WT14-5 and WT43-5) were challenged with the blast fungus using the press-injured spots method. The average size of disease lesions of the transgenic line WT43-5 was reduced to about half of that of non-transgenic plants. The 5-kDa peptide, corresponding to the processed form of the wasabi defensin, was detected in the total protein fraction extracted from the T3 progeny. Transgenic rice plants overproducing wasabi defensin are expected to possess a durable and wide-spectrum resistance (i.e. field resistance) against various rice blast races.

引用

页码：809 / 814

页数：6

共 21 条

[1]

BROEKAERT WF, 1995, PLANT PHYSIOL, V108, P1353, DOI [10.1016/j.coelec.2021.100721, 10.1016/j.chiabu.2021.105188]

[2] Inheritance, expression, and silencing of a chitinase transgene in rice [J].