Sialylated Milk Oligosaccharides Promote Microbiota-Dependent Growth in Models of Infant Undernutrition

被引：526

作者：

Charbonneau, Mark R. ^{[1
,2
]}

O'Donnell, David ^{[1
,2
]}

Blanton, Laura V. ^{[1
,2
]}

Totten, Sarah M. ^{[4
,5
]}

Davis, Jasmine C. C. ^{[4
,5
]}

Barratt, Michael J. ^{[1
,2
]}

Cheng, Jiye ^{[1
,2
]}

Guruge, Janaki ^{[1
,2
]}

Talcott, Michael ^{[3
]}

Bain, James R. ^{[8
,9
,10
,11
]}

Muehlbauer, Michael J. ^{[8
,9
]}

Ilkayeva, Olga ^{[8
,9
]}

Wu, Chao ^{[12
]}

Struckmeyer, Tedd ^{[12
]}

Barile, Daniela ^{[5
,6
]}

Mangani, Charles ^{[13
]}

Jorgensen, Josh ^{[7
]}

Fan, Yue-mei ^{[14
]}

Maleta, Kenneth ^{[13
]}

Dewey, Kathryn G. ^{[7
]}

Ashorn, Per ^{[14
,15
]}

Newgard, Christopher B. ^{[8
,9
,10
,11
]}

Lebrilla, Carlito ^{[4
,5
]}

Mills, David A. ^{[5
,6
]}

Gordon, Jeffrey I. ^{[1
,2
]}

机构：

[1] Washington Univ, Sch Med, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Ctr Gut Microbiome & Nutr Res, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Div Comparat Med, St Louis, MO 63110 USA

[4] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[5] Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA

[6] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA

[7] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA

[8] Duke Univ, Med Ctr, Sarah W Stedman Nutr & Metab Ctr, Durham, NC 27710 USA

[9] Duke Univ, Med Ctr, Duke Mol Physiol Inst, Durham, NC 27710 USA

[10] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[11] Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA

[12] Hilmar Cheese Co, Hilmar Ingredients, Hilmar, CA 95324 USA

[13] Univ Malawi, Coll Med, Blantyre 3, Malawi

[14] Univ Tampere, Sch Med, Dept Int Hlth, FI-33521 Tampere, Finland

[15] Tampere Univ Hosp, Dept Paediat, FI-33521 Tampere, Finland

来源：

CELL | 2016年 / 164卷 / 05期

基金：

芬兰科学院;

关键词：

SIALIC-ACID; CHILD UNDERNUTRITION; BONE; BIFIDOBACTERIA; MALNUTRITION; INSULIN; MASS; PIG;

D O I：

10.1016/j.cell.2016.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Identifying interventions that more effectively promote healthy growth of children with undernutrition is a pressing global health goal. Analysis of human milk oligosaccharides (HMOs) from 6-month-post-partum mothers in two Malawian birth cohorts revealed that sialylated HMOs are significantly less abundant in those with severely stunted infants. To explore this association, we colonized young germ-free mice with a consortium of bacterial strains cultured from the fecal microbiota of a 6-month-old stunted Malawian infant and fed recipient animals a prototypic Malawian diet with or without purified sialylated bovine milk oligosaccharides (S-BMO). S-BMO produced a microbiotadependent augmentation of lean body mass gain, changed bone morphology, and altered liver, muscle, and brain metabolism in ways indicative of a greater ability to utilize nutrients for anabolism. These effects were also documented in gnotobiotic piglets using the same consortium and Malawian diet. These preclinical models indicate a causal, microbiota-dependent relationship between S-BMO and growth promotion.

引用

页码：859 / 871

页数：13

共 43 条

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Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures [J].