Effect of berberine on barrier function in a human retinal pigment epithelial cell line

被引：33

作者：

Cui, Hu-Shan

Hayasaka, Seiji

Zhang, Xue-Yun

Hayasaka, Yoriko

Chi, Zai-Long

Zheng, Lian-Shun

机构：

[1] Toyama Univ, Dept Ophthalmol, Toyama 9300194, Japan

[2] Toyama Univ, Dept Pathol, Toyama 9300194, Japan

来源：

JAPANESE JOURNAL OF OPHTHALMOLOGY | 2007年 / 51卷 / 01期

关键词：

ARPE-19; cells; berberine; interleukin-1; beta; barrier function;

D O I：

10.1007/s10384-006-0395-0

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Purpose: To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1 beta (IL-1 beta). Methods: ARPE-19 cells were cultured to confluence. Berberine and IL-1 beta were added to the medium. Barrier functions were evaluated by measuring transepithelial electrical resistance (TER) and the permeability to horseradish peroxidase (HRP) and sodium fluorescein (SF). Results: Berberine dose-dependently inhibited decreased TER and increased the permeability to HRP and SF in the cells stimulated with IL-1 beta. Conclusions: Berberine dose-dependently inhibited the disruption of the barrier function in the ARPE-19 cell line induced by IL-1 beta.

引用

页码：64 / 67

页数：4

共 5 条

[1]

Interleukin-1β and barrier function of retinal pigment epithelial cells (ARPE-19):: Aberrant expression of junctional complex molecules [J].