Sensing of DNA non-homology lowers the initiation of meiotic recombination in yeast

被引:50
作者
Rocco, V [1 ]
Nicolas, A [1 ]
机构
[1] INST CURIE, SECT RECH, UMR144 CNRS, F-75248 PARIS 05, FRANCE
关键词
D O I
10.1046/j.1365-2443.1996.00256.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background: Meiotic recombination between homologous chromosomes in the yeast Saccharomyces cerevisiae is initiated by the formation of DNA double-strand breaks (DSBs). The mechanism of DSB formation and the factors that determine their frequency and location have yet to be elucidated. Current studies of meiotic recombination are also concerned with the question of the functional relationship between DSB formation and the other meiotic processes of homology searching, pairing and synapsis of homologues. Results: To test if DNA identity is required for high levels of DSBs and recombination, we have asked DNA heterologies (140-547bp) the well characterized ARG4 of meiotic recombination, can affect DSB formation and gene conversion events in the ARG4 locus. The present physical and genetic analyses show that some heterologies reduced recombination frequencies without altering DSB formation, whereas others reduced both DSB and gene conversion frequencies. Conclusions: These results suggest that DNA heterologies overlapping a recombination initiator impair meiotic gene conversion at two levels, First, some heterologies affect the level of DSB formation, revealing the existence of an anti-initiation process sensing the presence of sequence non-homology between the homologous chromosomes, Second, heterologies can impair the successful processing of the recombination intermediates once DSBs are made, We present a model for interhomologue cross-talks involving chromosomal and DNA/DNA interactions.
引用
收藏
页码:645 / 661
页数:17
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