Analysis of the catalytic domain of phosphatidylinositol 4-kinase type II

被引:33
作者
Barylko, B
Wlodarski, P
Binns, DD
Gerber, SH
Earnest, S
Sudhof, TC
Grichine, N
Albanesi, JP
机构
[1] Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA
[2] Univ Texas, SW Med Ctr, Dept Mol Genet, Dallas, TX 75390 USA
[3] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75390 USA
[4] Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Dallas, TX 75390 USA
[5] Med Univ Warsaw, Dept Histol & Embryol, Ctr Biostruct Res, PL-02004 Warsaw, Poland
关键词
D O I
10.1074/jbc.M203241200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phosphatidylinositol (PtdIns) 4-kinases catalyze the conversion of PtdIns to PtdIns 4-phosphate, the major precursor of phosphoinositides that regulates a vast array of cellular processes. Based on enzymatic differences, two classes of PtdIns 4-kinase have been distinguished termed Types 11 and Ill. Type III kinases, which belong to the phosphatidylinositol (PI) 3/4-kinase family, have been extensively characterized. In contrast, little is known about the Type 11 enzymes (PI4KIIs), which have been cloned and sequenced very recently. PI4KIIs bear essentially no sequence similarity to other protein or lipid kinases; hence, they represent a novel and distinct branch of the kinase superfamily. Here we define the minimal catalytic domain of a rat PI4KII isoform, PI4KIIalpha, and identify conserved amino acid residues required for catalysis. We further show that the catalytic domain by itself determines targeting of the kinase to membrane rafts. To verify that the PI4KII family extends beyond mammalian sources, we expressed and characterized Drosophila PI4KII and its catalytic domain. Depletion of PI4KII from Drosophila cells resulted in a severe reduction of PtdIns 4-kinase activity, suggesting the in vivo importance of this enzyme.
引用
收藏
页码:44366 / 44375
页数:10
相关论文
共 55 条
[1]   The genome sequence of Drosophila melanogaster [J].
Adams, MD ;
Celniker, SE ;
Holt, RA ;
Evans, CA ;
Gocayne, JD ;
Amanatides, PG ;
Scherer, SE ;
Li, PW ;
Hoskins, RA ;
Galle, RF ;
George, RA ;
Lewis, SE ;
Richards, S ;
Ashburner, M ;
Henderson, SN ;
Sutton, GG ;
Wortman, JR ;
Yandell, MD ;
Zhang, Q ;
Chen, LX ;
Brandon, RC ;
Rogers, YHC ;
Blazej, RG ;
Champe, M ;
Pfeiffer, BD ;
Wan, KH ;
Doyle, C ;
Baxter, EG ;
Helt, G ;
Nelson, CR ;
Miklos, GLG ;
Abril, JF ;
Agbayani, A ;
An, HJ ;
Andrews-Pfannkoch, C ;
Baldwin, D ;
Ballew, RM ;
Basu, A ;
Baxendale, J ;
Bayraktaroglu, L ;
Beasley, EM ;
Beeson, KY ;
Benos, PV ;
Berman, BP ;
Bhandari, D ;
Bolshakov, S ;
Borkova, D ;
Botchan, MR ;
Bouck, J ;
Brokstein, P .
SCIENCE, 2000, 287 (5461) :2185-2195
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments [J].
Balla, A ;
Tuymetova, G ;
Barshishat, M ;
Geiszt, M ;
Balla, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (22) :20041-20050
[4]   Phosphatidylinositol 4-kinases [J].
Balla, T .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1998, 1436 (1-2) :69-85
[5]   Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinases [J].
Balla, T ;
Downing, GJ ;
Jaffe, H ;
Kim, S ;
Zolyomi, A ;
Catt, KJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (29) :18358-18366
[6]   A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans [J].
Barylko, B ;
Gerber, SH ;
Binns, DD ;
Grichine, N ;
Khvotchev, M ;
Südhof, TC ;
Albanesi, JP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :7705-7708
[7]   Bifurcation of lipid and protein kinase signals of PI3Kγ to the protein kinases PKB and MAPK [J].
Bondeva, T ;
Pirola, L ;
Bulgarelli-Leva, G ;
Rubio, I ;
Wetzker, R ;
Wymann, MP .
SCIENCE, 1998, 282 (5387) :293-296
[8]  
Brill JA, 2000, DEVELOPMENT, V127, P3855
[9]   PHOSPHOINOSITIDE KINASES [J].
CARPENTER, CL ;
CANTLEY, LC .
BIOCHEMISTRY, 1990, 29 (51) :11147-11156
[10]   Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways [J].
Clemens, JC ;
Worby, CA ;
Simonson-Leff, N ;
Muda, M ;
Maehama, T ;
Hemmings, BA ;
Dixon, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6499-6503