Analysis of the distribution of Cu, Fe and Zn and other elements in brindled mouse kidney using a scanning proton microprobe

被引：15

作者：

Kirby, BJ

Danks, DM

Legge, GJF

Mercer, JFB

机构：

[1] Univ Melbourne, Sch Phys, Microanalyt Res Ctr, Parkville, Vic 3052, Australia

[2] Royal Childrens Hosp, Murdoch Inst, Parkville, Vic 3052, Australia

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1998年 / 71卷 / 3-4期

基金：

澳大利亚研究理事会;

关键词：

Menkes disease; copper distribution; proton microprobe; mottled mice; elemental analysis; trace elements; microanalysis; brindled mouse; kidney; proximal tubule; distal tubule; copper transport;

D O I：

10.1016/S0162-0134(98)10053-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An analysis of the distribution of trace metals in the kidney cortex in normal and brindled male mice has been carried out with a scanning proton microprobe. Enzyme histochemical staining techniques were used to distinguish between proximal and distal tubules. Average copper levels were increased in brindled kidney tissue sections, with the above-normal Cu accumulation found to occur entirely within the proximal tubules. Therefore, the proximal tubule is now regarded as the location where the defect in Cu transport in brindled mice is manifested the most clearly. The distribution of Fe was found to be non-uniform with some tubule cross-sections exhibiting high concentrations in both genotypes. The distribution of Zn was found to be uniform, and the concentration was similar for each genotype. (C) 1998 Elsevier Science Inc. All rights reserved.

引用

页码：189 / 197

页数：9

共 38 条

[1] COPPER-METABOLISM IN MOTTLED MOUSE MUTANTS - COPPER CONCENTRATIONS IN TISSUES DURING DEVELOPMENT [J].